T淋巴瘤侵袭转移诱导因子1在膀胱癌组织中的表达及其与膀胱癌复发的关系

Expression of T-lymphoma invasion and metastasis-inducing factor 1 in bladder cancer tissue and its relationship with recurrence of bladder cancer

目的探讨T淋巴瘤侵袭转移诱导因子1(Tiam1)在膀胱癌组织中的表达及其与膀胱癌复发的关系。方法选择50例正常膀胱及233例膀胱癌组织,采用免疫组化、实时定量聚合酶链式反应、蛋白免疫印迹法分别检测Tiam1蛋白表达阳性及Tiam1mRNA、Tiam1蛋白相对表达情况,并分析膀胱癌Tiam1蛋白表达阳性与阴性患者的病理特征。结果膀胱癌组织中的Tiam1蛋白表达阳性率及Tiam1mRNA、Tiam1蛋白相对表达量均高于正常膀胱癌组织(均P<0.05)。膀胱癌Tiam1蛋白阳性表达组和Tiam1蛋白阴性表达组的病理分级、病理分期、淋巴结转移及复发率比较,差异均有统计学意义(均P<0.05);Cox回归分析结果显示,肿瘤高分级、病理高分期、淋巴结转移阳性及Tiam1蛋白表达阳性是膀胱癌患者复发的危险因素(均P<0.05)。结论Tiam1在膀胱癌中表达升高,且Tiam1蛋白表达阳性与膀胱癌复发密切相关。

Objective To investigate the expression of T-lymphoma invasion and metastasis-inducing factor 1(Tiam 1) in bladder cancer tissue and its relationship with recurrence of bladder cancer. Methods Fifty cases of normal bladder tissue and 233 cases of bladder cancer tissue were selected.The positive rate of Tiam1 protein expression and the relative expression of Tiam1 mRNA and protein were detected by immunohistochemistry,real-time quantitative PCR and Western blot,respectively.The pathological characteristics of patients with positive or negative expression of Tiam1 protein in bladder cancer were analyzed. Results The positive rate of Tiam1 protein expression and the relative expression of Tiam1 mRNA and protein in bladder cancer tissue were higher than those in normal bladder tissue(all P <0.05).There were statistically significant differences in pathological grade,pathological stage,lymph node metastasis,and recurrence rate between the positive expression Tiam1 protein group and the negative expression Tiam1 protein group in bladder cancer(all P <0.05);Cox regression analysis results showed that high tumor grade,high tumor stage,positive lymph node metastasis,and positive expression of Tiam1 protein were risk factors for recurrence of bladder cancer(all P <0.05). Conclusion Elevated Tiam1 expression is observed in bladder cancer,and the positive expression of Tiam1 protein is closely related to the recurrence of bladder cancer.