克罗米芬促排卵周期中应用寿胎丸对小鼠着床窗口期子宫内膜及其容受性、妊娠结局的影响

Effect of Shoutai Pill used in clomiphene ovulatory cycle on endometrium and its receptivity during implantation window period and pregnancy outcome in mice

目的探讨克罗米芬促排卵周期中应用寿胎丸对小鼠着床窗口期子宫内膜及其容受性、妊娠结局的影响。方法将90只小鼠随机分为对照组、克罗米芬组、中药组,每组30只。对照组小鼠给予生理盐水灌胃,克罗米芬组、中药组小鼠均给予克罗米芬灌胃。2d后,3组小鼠均给予腹腔内注射人绒毛膜促性腺激素5IU并合笼,对照组小鼠继续给予生理盐水灌胃,克罗米芬组、中药组分别灌胃生理盐水、寿胎丸。至合笼后第4天,每组各处死10只小鼠,记录妊娠情况,并观察子宫内膜形态、厚度,检测白血病抑制因子(LIF)蛋白及mRNA表达。分别于合笼后第5天、第7天,每组各处死10只小鼠,计算总妊娠率。结果合笼后第4天,与其他两组比较,克罗米芬组子宫内膜厚度变薄,腺体周长、面积和最大直径减少,且子宫内膜LIFmRNA及蛋白的表达水平均降低(均P<0.05),而中药组与对照组差异无统计学意义(P>0.05)。镜下可见克罗米芬组子宫内膜腺体排列稀疏,腺上皮呈低柱状,间质致密,部分腺腔内见空泡;中药组子宫内膜腺体腺上皮呈高柱状,腺腔内见较多空泡,间质疏松,血管延长,腺体数目及成熟度接近对照组。克罗米芬组的总妊娠率低于其他两组(P<0.05),而中药组与对照组差异无统计学意义(P>0.05)。结论克罗米芬促排卵周期中应用寿胎丸,有利于改善着床窗口期子宫内膜厚度及容受性,从而提高妊娠率。

Objective To investigate the effect of Shoutai Pill used during implantation window period on endometrium and its endometrial receptivity in clomiphene ovulatory cycle and pregnancy outcome in mice. Methods Ninety mice were randomly divided into control group,clomiphene group and Chinese medicine group,with 30 mice in each group.The control group was given normal saline by gavage,the clomiphene group and the Chinese medicine group were both given clomiphene by gavage.Two days later,the three group received intraperitoneal injection of 5 IU human chorionic gonadotropin and cohabitating,then the control group was given intragastric administration of normal saline continuously,the clomiphene group and the Chinese medicine group were given intragastric administration of normal saline and Shoutai Pill,respectively.On the 4th day after cohabitating,10 mice were killed in each group to record conditions of pregnancy,observe endometrial morphology and thickness,and detect leukemia inhibitory factor(LIF) protein and mRNA expression. Ten mice were killed in each group on the 5th day and 7th day,respectively, after cohabitating,and total pregnancy rate was calculated. Results On the 4th day after cohabitating,the clomiphene group exhibited decreased endometrial thickness,decreased perimeter,area and maximum diameter of the gland,and lower expression levels of endometrial LIF mRNA and protein as compared with the other two groups(all P <0.05),furthermore,there was no statistically significant difference between the Chinese medicine group and the control group ( P >0.05).Microscopically,endometrial glands were sparse and exhibited low columnar epithelium and dense stroma in the clomiphene group,with visible vacuoles inside some lumens;endometrial glands in the Chinese medicine group appeared with high columnar epithelium,more visible vacuoles inside lumens,loose stroma,and prolonged blood vessels ,with number and maturity close to the controls.The total pregnancy rate in the clomiphene group was lower than that in the other two groups( P <0.05),moreover,no statistically significant difference was found between the Chinese medicine group and the control group( P >0.05). Conclusion Shoutai Pill used in clomiphene ovulation cycle is beneficial to improvement in endometrial thickness and receptivity during implantation window period,thus increasing pregnancy rate.