基质金属蛋白酶-9对出生后小鼠骨发育和骨改建的作用研究

Effects of Matrix Metalloproteinase-9 on Bone Development and Remodeling of Postnatal Mice

目的:本研究旨在探讨基质金属蛋白酶-9(matrix metalloproteinase-9,MMP-9)在出生后小鼠股骨与颅面骨发育与改建过程中的作用。方法:使用X射线投射技术、Micro-CT扫描及三维成像技术、组织学染色技术,对比分析MMP-9基因敲除(MMP-9^-/-)小鼠与野生型小鼠在不同发育阶段骨组织的特征。结果:较之野生型小鼠,MMP-9-/-小鼠的股骨骨小梁厚度明显低于野生型小鼠。12周MMP-9^-/-小鼠的骨髓腔中存在大量脂肪细胞。MMP-9^-/-小鼠颅面骨矿化程度低于野生型小鼠。结论:MMP-9基因缺失抑制了小鼠股骨与颅面骨的发育与改建,提示MMP-9在骨发育与骨稳态过程中发挥重要作用。

Objective: To investigate the effect of matrix metalloproteinase-9 (MMP-9) on the development and remodeling of femur and craniofacial bone in postnatal mice.Methods: X-ray projection,Micro-CT scanning,three-dimensional imaging,and histological staining were used to observe and analyze the characteristics of bone of MMP-9^-/- mice with wild-type mice at different developmental stages.Results: Compared with the wild-type mice,the femoral trabecular thickness of adult mice was lower than that of wild-type mice.Fat cells were abundant in the bone marrow cavity at 12-week-old MMP-9^-/- mice.The degree of skull mineral density in MMP-9^-/- mice was lower than that of normal mice.Conclusion: MMP-9 gene deletion inhibited the development and remodeling of femoral and craniofacial bones in mice,suggesting that MMP-9 plays an important role in bone development and bone homeostasis.