硅酸钙的掺入对胶原-壳聚糖支架的影响

Effect of Calcium Silicate Incorporation on Collagen-chitosan Scaffold

目的:探索不同质量分数的硅酸钙对胶原-壳聚糖支架性能的影响。方法:通过冷冻干燥法制成硅酸钙含量分别为0%、1%、2%、5%和8%(w/v)的支架,采用扫描电镜、能量色散谱分析,以及万能试验机检测其理化性能的变化。通过模拟体外矿化和细胞培养,检测其生物学性能的变化。结果:与对照组(0%w/v硅酸钙)比较,随着硅酸钙含量的增加,支架的压缩弹性模量逐渐增加(P<0.05),降解速率也逐渐增加(P<0.05),而孔隙率逐渐减小(P<0.05);实验组均能促进成骨细胞的增殖以及碱性磷酸酶(alkaline phosphatase,ALP)的表达(P<0.05)。此外,实验组均有不同程度的羟基磷灰石晶体沉积,而对照组几乎无羟基磷灰石晶体沉积。结论:适量的硅酸钙能增强胶原-壳聚糖支架的理化性能,促进成骨及体外矿化,有作为骨组织工程支架的潜力。

Objective: To explore the effect of calcium silicate incorporation on collagen-chitosan scaffold.Methods: The scaffolds with 0%,1%,2%,5%,and 8%(w/v) calcium silicate were prepared by freeze-drying method.The physicochemical properties of the scaffolds were tested by scanning electron microscopy,energy dispersive spectroscopy,and universal testing machine.The changes of biological properties were detected by simulating mineralization and cell culture in vitro.Results: With the increase of calcium silicate,the compressive elastic modulus and degradation rate of the scaffolds increased while the porosity decreased gradually compared to the control group (0% calcium silicate,P <0.05).The experimental group could promote the proliferation of osteoblasts and the expression of ALP ( P <0.05).Moreover,varying amount of HA crystals were deposited in different experimental groups,while no HA crystal was deposited in the control group.Conclusion: Appropriate amount of calcium silicate can enhance the physicochemical properties of collagen-chitosan scaffold and promote osteogenesis and mineralization in vitro suggesting a potential scaffold for bone tissue engineering.