ATR-FTIR技术在赛加羚羊角鉴别中的应用分析

Application Analysis of ATR-FTIR Technology in Antelope Horn Identification

利用衰减全反射红外光谱技术,对赛加羚羊等3个物种共49份相关样本进行检测、分析并计算其相关系数,结果:同支羚羊角壳不同取样部位红外光谱相关系数为96.69%—99.43%;不同羚羊角壳相同取样部位红外光谱相关系数为94.24%—99.43%;不同物种角质壳红外光谱相关系数为99.28%—99.47%;羚羊角壳与其骨质芯混合物红外光谱随角骨比例变化,骨质成分越多,其红外光谱与羚羊角壳红外光谱的相关系数越低。由此推断红外光谱因取样部位、个体及物种不同而产生差异,且个体间和取样部位差异甚至大于种间差异,而骨质成分的混入则会对羚羊角的红外光谱产生不可预判的影响,显示ATR-FTIR技术应用于羚羊角的鉴别应十分慎重。

We analyzed 49 horn samples of saiga antelope ( Saiga tatarica ),Mongolian gazelle ( Procapra gutturosa ),and cattle ( Bos taurus ) using attenuated total reflectance Fourier transform infrared spectroscopy ( ATR-FTIR ),and calculated the correlation coefficients.The correlation coefficient of the infrared spectrum of different sampling sites of a saiga antelope horn shell was between 96.43% and 99.69%.The correlation coefficient of infrared spectrum of the same sampling site of different saiga antelope horn shell was between 94.24% and 99.43%.The correlation coefficients of the horn shell of different species were between 99.28% and 99.47%. The infrared spectrum of the mixture of antelope horn shell and bone core changed with the proportion of horn bone.The more bone components,the lower the correlation coefficient between its infrared spectrum and the infrared spectrum of the saiga antelope horn shell.We conclude that individual differences,sampling sites,bone-horn ratios,and other species composition analogs affect the infrared spectrum identification of antelope horns,so we should be cautious when using ATR-FTIR to identify antelope horns.