小檗碱对5种临床常见念珠菌体外抑菌作用分析

Inhibitory effect of berberine in vitro against clinical common Candida

目的检测小檗碱对5种临床常见念珠菌的抑菌效果。方法收集分离自临床样本的56株念株菌(白念珠菌23株、光滑念珠菌20株、热带念珠菌6株、克柔念珠菌4株、近平滑念珠菌3株),采用微量肉汤稀释法对其进行体外药物敏感性试验。结果小檗碱对白念珠菌、光滑念珠菌、热带念珠菌、克柔念珠菌及近平滑念珠菌的最小抑菌浓度(MIC)50分别为32、16、8、4和16μg/mL,对白念珠菌的抑菌效果与其他4种念珠菌比较差异均有统计学意义(P<0.05),小檗碱对光滑念珠菌、热带念珠菌、克柔念珠菌的抑菌效果比较差异无统计学意义(P>0.05),小檗碱对敏感及非敏感光滑念珠菌的抑菌效果差异亦无统计学意义(P=0.063)。结论小檗碱对临床常见的5种念珠菌(包括非敏感光滑念珠菌)均有较好的抑菌效果,对非白念珠菌的抑菌效果优于白念珠菌。

Objective To determine the inhibitory effect of berberine to 5 kinds of clinical common Candida.Methods A total of 56 Candida isolates were collected.Among them,23 isolates were Candida albicans,20 isolates were Candida glabrata,6 isolates were Candida tropicalis,4 isolates were Candida kruseii,and 3 isolates were Candida parapsilosis.Broth micro-dilution method was used to determine the minimum inhibitory concentration(MIC)of berberine to 5 kinds of clinical common Candida.Results The MIC50 of berberine against Candida albicans,Candida glabrata,Candida tropicalis,Candida kruseii and Candida parapsilosis were 32,16,8,4 and 16μg/mL,respectively.The inhibitory effect of berberine on Candida albicans had statistical significance compared with the other 4 kinds of Candida(P<0.05),while there was no statistical significance among the other 4 kinds of Candida(P>0.05).There was no statistical significance between sensitive group and non-sensitive group of Candida glabrata(P=0.063).Conclusions Berberine has a good inhibitory effect against the 5 kinds of clinical common Candida,including non-sensitive Candida glabrata,while the inhibitory effect against non-Candida albicans is better than that against Candida albicans.