口腔鳞状细胞癌组织中VASH-1、LGR5的表达及相关性分析

Expression and correlation of VASH-1 and LGR5 in oral squamous cell carcinoma

目的分析口腔鳞状细胞癌组织中血管生成抑制蛋白-1(VASH-1)、富含亮氨酸重复序列G-蛋白偶联受体5(LGR5)的表达及临床意义。方法采用免疫组织化学SP法对2016年3月至2018年3月唐山市协和医院收治的87例口腔鳞状细胞癌患者癌组织及癌旁组织中VASH-1、LGR5的表达情况进行检测,分析VASH-1、LGR5表达与口腔鳞状细胞癌患者临床病理特征的关系,并分析口腔鳞状细胞癌组织中VASH-1、LGR5表达的相关性。结果口腔鳞状细胞癌组织中VASH-1阳性率、LGR5阳性率均显著高于癌旁组织(P<0.05)。病理分级为Ⅰ级、肿瘤直径小于等于2cm、TNM分期为Ⅰ~Ⅱ期、无淋巴结转移的口腔鳞状细胞癌患者VASH-1阳性率、LGR5阳性率均显著低于病理分级为Ⅱ~Ⅲ级、肿瘤直径大于2cm、TNM分期为Ⅲ~Ⅳ期、淋巴结转移患者(P<0.05)。经Spearman相关性分析显示:口腔鳞状细胞癌组织中VASH-1表达和LGR5表达呈正相关(Rs=0.723,P=0.000)。结论口腔鳞状细胞癌组织中VASH-1、LGR5呈明显高表达,其水平与肿瘤直径、病理分级、淋巴结转移存在密切相关性,且VASH-1表达和LGR5表达呈正相关。

Objective To analyze the expressions and clinical significance of vasohibin 1(VASH-1) and leucine-rich repeat-containing G-protein-coupled receptor 5 (LGR5) in oral squamous cell carcinoma(OSCC). Methods The expressions of VASH-1 and LGR5 in cancer tissues and adjacent tissues of 87 patients with oral squamous cell carcinoma treated in Tangshan union medical college hospital from March 2016 to March 2018 were detected by immunohistochemical SP method.The relationship between the expressions of VASH-1 and LGR5 and the clinicopathological features of oral squamous cell carcinoma patients was analyzed,and the correlation between VASH-1 and LGR5 expression in oral squamous cell carcinoma tissues was analyzed. Results The positive rates of VASH-1 and LGR5 in oral squamous cell carcinoma tissues were significantly higher than those in adjacent tissues( P <0.05).The positive rates of VASH-1 and LGR-5 of patients with pathological grade Ⅰ,tumor diameter≤2 cm,TNM stage Ⅰ to Ⅱ,and without lymph node metastasis were significantly lower than those of patients with pathological grade Ⅱ to Ⅲ,tumor diameter>2 cm,TNM stage Ⅳ to Ⅳ,and had lymph node metastasis( P <0.05).Spearman correlation showed that there was a positive correlation between the expressions of VASH-1 and LGR5 in oral squamous cell carcinoma ( Rs =0.723, P =0.000 ). Conclusion The expressions of VASH-1 and LGR5 in oral squamous cell carcinoma are significantly higher,and the levels of VASH-1 and LGR5 are closely related to tumor diameter,pathological grade and lymph node metastasis,and the expression of VASH-1 is positively correlated with the expression of LGR5.