基于光学相干层析成像的皮层血流高分辨率成像

High-Resolution Cortical Blood Flow Imaging Based on Optical Coherence Tomography

光学相干层析成像(OCT)技术具有非接触、分辨率高、采集速度快等优势,不仅能够显示样品的三维结构,而且能够检测样品中的运动信息。构建了高分辨率光谱OCT血管成像系统,系统横向分辨率约为6.7μm,纵向分辨率约为4.7μm,相机线采集速度140kHz,能够在2s内完成三维扫描。在此基础上,通过相邻B扫描图像差分运算提取血流信息,实现血管成像。应用该系统扫描了大鼠大脑皮层的三维血管网络,实验结果表明,系统具有对毛细血管成像的能力。

Optical coherence tomography(OCT)is a non-contact imaging modality with advantages of high resolution and high acquisition speed.OCT can provide three-dimensional image structures and detect motion information in the samples.In this study,a high-resolution spectral OCT angiography system was constructed with the lateral and axial resolutions of about 6.7μm and about 4.7μm,respectively.The line scan camera was operated at 140kHz and could complete a three-dimensional scan within 2s.Based on this,the blood flow perfusion information was extracted by differentiating the adjacent B-scan images.A micro-vasculature network image of rat cerebral cortex was obtained.The experimental results demonstrate that the proposed system is suitable for capillary imaging.