融合肽TAT-Helicokinin I对舞毒蛾生物活性研究

Bioactivity of TAT-Helicokinin I fusion peptide to larvae of Lymantria dispar

以美洲棉铃虫Helicoverpa zea利尿激肽Helicokinin I和穿膜肽TAT为基础合成融合肽TAT-Helicokinin I,并测试3种肽对舞毒蛾Lymantria dispar幼虫的注射活性;探究融合肽TAT-Helicokinin I和利尿激肽Helicokinin I经取食途径进入虫体后对舞毒蛾幼虫生长的影响。结果表明:TAT-Helicokinin I和Helicokinin I对舞毒蛾幼虫均表现出注射毒性,且TAT-Helicokinin I比Helicokinin I表现出更高的生物活性;60,600 pmol/头的剂量对舞毒蛾幼虫均表现出显著致死性,6 pmol/头的剂量对舞毒蛾幼虫生长无影响;Helicokinin I饲喂舞毒蛾幼虫未显现出毒性,而饲喂TAT-Helicokinin I对试虫生长表现出显著抑制作用。该研究证实TAT与Helicokinin I融合后能够降低昆虫消化道酶对利尿激肽Helicokinin I的降解。

The peptide of Helicokinin I from Helicoverpa zea was synthesized as a fusion peptide with a transactivator of transcription(TAT)protein.Bioactivity of fusion peptide of TAT-Helicokinin I to larvae of gypsy moth was evaluated by injection and feeding.Results indicated that Lymantria dispar larvae injected TAT-Helicokinin I or Helicokinin I exhibited a significantly reduced survival rate.TAT-Helicokinin I showed greater bioactivity than Helicokinin I.The dose of 60 and 600 pmol showed significant lethality to test larvae.The dose of 6 pmol showed no lethality to test larvae.TAT-Helicokinin I was spread on the artificial diet,and its growth-inhibiting effect was determined.However,single peptide of Helicokinin I did not show toxicity to larvae of gypsy moth by feeding.The fusion peptide TAT-Helicokinin I showed toxicity to gypsy moth larvae in injection and feeding test.The results indicated that the fusion for TAT and Helicokinin can reduce the degradation by insect digestive tract efficiently.