长链非编码RNA DEANR1等在肺癌中的表达

Expression of long non-coding RNA DEANR1 and others in lung cancer

目的探讨长链非编码RNA(lncRNA)DEANR1、INXS、ANCR、FENDRR等在肺癌中的表达。方法选取2018年1~6月昆明医科大学第三附属医院收治的54例肺癌患者,其中鳞癌、腺癌、肺小细胞癌分别为28、22、4例。经手术获取癌组织、癌旁正常组织、肺癌淋巴结转移组织,提取总RNA,并采用SYBR Green实时荧光定量PCR检测基因的RNA表达量。结果 DEANR1、INXS、ANCR在癌组织中的相对表达量均高于癌旁正常组织(P <0.05)。淋巴结组织中ANCR、INXS的相对表达量与ki-67呈正相关(r=0.757,P=0.049;r=0.802,P=0.030)。结论 DEANR1、INXS、ANCR在癌组织中的相对表达量均升高,可能在肺癌的发生发展中起到促进作用,ANCR、INXS在淋巴结中的表达与ki-67呈正相关,可能与肺癌淋巴结转移及不良预后有关。

Objective To investigate the expression of long non-coding RNA(lncRNA) DEANR1, INXS, ANCR, FENDRR in lung cancer. Methods The study included 54 patients with lung cancer admitted to the Third Affiliated Hospital of Kunming Medical University from January to June 2018, including squamous cell carcinoma, adenocarcinoma, and small cell lung cancer in 28, 22, 4 cases, respectively. The cancer tissue, the lymph node metastasis and the tissue adjacent to carcinoma of 54 cases of patients with lung cancer was used to detect the expression of these genes by the real-time fluorescence quantitative PCR. Results The expression levels of INXS and ANCR in cancer tissues were higher than those in adjacent normal tissues(P < 0.05). The expression levels of ANCR and INXS in lymph node tissues were positively correlated with ki-67(r = 0.757, P = 0.049;r = 0.802, P = 0.030). Conclusion The increased expression of DEANR1, INXS and ANCR in cancer tissues may be a positive factor in the development of lung cancer. The expression of ANCR and INXS in lymph nodes is positively correlated with ki-67, which may be related to lymph node metastasis and poor prognosis of lung cancer.