胆汁淤积性肝病婴儿粪便中细菌相关代谢产物短链脂肪酸水平的分析

Levels of short-chain fatty acids in enterobacteria-related metabolites in the feces of infants with cholestatic hepatopathy

目的比较婴儿胆汁淤积性肝病(ICH)患儿和健康婴儿粪便中细菌相关代谢产物短链脂肪酸的差异,并探讨其临床意义.方法选取诊断为ICH患儿30例,另选取健康婴儿30例为对照组.收集ICH患儿治疗前组、治疗后组和对照组的粪便标本.应用气相色谱技术定量测定各组婴儿粪便中短链脂肪酸包括乙酸、丙酸、丁酸、异丁酸、异戊酸的含量,并进行比较.结果对照组、治疗前组、治疗后组的乙酸、丙酸浓度差异无统计学意义(P>0.05),治疗后组丁酸浓度较治疗前组升高(P<0.05).对照组异丁酸、异戊酸浓度高于治疗前组及治疗后组(P<0.05).结论 ICH患儿的肠道蛋白质代谢产物与正常婴儿存在差异,而碳水化合物代谢产物差异不显著.

Objective To compare the levels of short-chain fatty acids in enterobacteria-related metabolites in feces between infants with cholestatic hepatopathy and healthy infants. Methods Thirty infants with cholestatic hepatopathy were enrolled in this study as the disease group, while 30 healthy infants were enrolled as the control group. Fecal specimens were collected from the disease group before and after treatment and from the control group. Gas chromatography was used to quantitatively determine the content of short-chain fatty acids in the feces of both groups including acetic acid, propionic acid, butyric acid, isobutyric acid, and isovaleric acid. Results There were no significant differences in the concentrations of acetic acid and propionic acid between the control and disease groups before and after treatment, as well as no significant changes in the two markers in the disease group after treatment (P>0.05). The disease group had a significantly increased concentration of butyric acid after treatment (P<0.05). The concentrations of isobutyric acid and isovaleric acid in the control group were significantly higher than those in the disease group before and after treatment (P<0.05). Conclusions Intestinal protein metabolites in infants with cholestatic hepatopathy are significantly different from those in healthy infants, whereas there is no significant difference with respect to carbohydrate metabolites.