Cellavision DM96高级红细胞应用软件在裂红细胞分析中的应用评价

Value of Cellavision DM96 Advanced Red Blood Cell Software on schistocyte analysis

目的评价Cellavision DM96高级红细胞应用软件在裂红细胞检测方面应用价值。方法选取57例患者及60例健康人血液标本,分别用人工镜检及Cellavision DM96自动图像分析系统自动检测裂红细胞。结果与人工镜检相比,使用Cellavision DM96进行裂红细胞计数时,批内精密度、日间精密度及检测者间比对变异系数均较低。 Cellavision DM96自动检测灵敏度高,但特异度较差;人工修正后,与人工镜检有很好的一致性。Cellavision DM96自动检测裂红细胞均值较人工镜检略高。结论使用Cellavision DM96高级红细胞应用软件自动计数裂红细胞是一种精密度良好,灵敏度及特异度均符合临床需求,检测较准确,易于临床工作及教学使用的工具,有助于裂红细胞检测的标准化。

Objective To evaluate value of Cellavision DM96 Advanced Red Blood Cell (RBC) Software on schistocyte analysis.Methods A total of 57 samples of hospitalized patients and 60 samples of controls were analyzed.Schistocyte counts were evaluated with the reference manual microscopic method comparing the automated count on Cellavision DM96,both before and after reclassification.Results Compared with the manual microscopic count,within-run,between-run and between-observer coefficients of variation were lower when counted by Cellavision DM96.After reclassification,method comparison studies revealed good agreement with the manual microscopic method,with slightly higher values of schistocytes for the automated analysis.Conclusion The Cellavision Advanced RBC Software provides a sensitive and reproducible measurement of schistocyte in peripheral blood,but still requires manual revision in specificity.Further more,it is an easy-to-use software and an excellent teaching tool that might contribute to standardization in the investigation of schistocyte-related conditions.