质膜H+-ATPase对2种湿地植物吸收NO3-的影响

EFFECT OF PLASMA MEMBRANE H+-ATPase ON NO3- UPTAKE IN TWO WETLAND PLANTS

为研究湿地植物净化硝态氮(NO3^-)的效果及吸收机理,以凤眼莲、美人蕉2种常用湿地植物为实验材料,采用溶液培养法研究2种植物在模拟农田水体中对NO3^-的去除率与质膜H^+-ATPase活性及其与14-3-3蛋白的互作水平的关系。结果表明:凤眼莲对NO3^-的去除率高于美人蕉,其根尖质膜H^+-ATPase活性、叶片中硝酸还原酶活性均大于美人蕉。质膜H^+-ATPase活性影响植株NO3^-的吸收能力,表明质膜H^+-ATPase参与了植物吸收NO3^-的调控作用。

The aim of the experment was to study the absorption mechanism of nitrate nitrogen(NO3^-) in two species of wetland plants.A hydroponic experiment was conducted with Eichhornia Crassipes and Cana generalis Bailey to study plasma membrane H^+-ATPase(PM H^+-ATPase) activity and their interaction with 14-3-3 protein on NO3^- removal rate of two species of wetland plants in simulated farmland water.The results showed that the removal rate of NO3^- and PM H^+-ATPase activity in Eichhornia Crassipes was higher than those in Cana generalis Bailey,and activity of nitrate reductase in leaves was also higher than that of Cana generalis Bailey.The study revealed that higher activity of PM H^+-ATPase could enhance sequentially absorption of NO3^-,which indicated that PM H^+-ATPase was involved in regulation of NO3^- uptake in plants.