异甘草素甲苷的合成及抗氧化活性研究

Synthesis and Antioxidant Activity of Isoliquiritigenin Methyl Glucoside

以间苯二酚、冰醋酸、对甲氧基苯甲醛为原料,通过酰化、羟醛缩合反应合成异甘草素甲苷,通过~1HNMR、IR对其结构进行表征,利用其与DPPH自由基溶液的反应考察其抗氧化活性。结果表明,异甘草素标准品对DPPH自由基清除率为43.01%,异甘草素甲苷对DPPH自由基清除率为23.14%。异甘草素甲苷抗氧化活性低于异甘草素,故异甘草素的4位羟基是抗氧化活性有效部位。

Using resorcinol,glacial acetic acid,and p-methoxybenzaldehyde as raw materials,we synthesized isoliquiritigenin methyl glucoside by acylation and aldol condensation reactions,and characterized its structure by ~1HNMR and IR.Moreover,we investigated the antioxidant activity by the reaction of isoliquiritigenin methyl glucoside and DPPH free radical solution.The results show that the scavenging rates of DPPH free radical by reference substance isoliquiritigenin and isoliquiritigenin methyl glucoside are 43.01% and 23.14%,respectively.The antioxidant activity of isoliquiritigenin methyl glucoside is lower than that of isoliquiritigenin,so the 4-hydroxyl of isoliquiritigenin is the effective site for antioxidant activity.