3-O-C12-HSL通过PPARγ拮抗树突状细胞表达RP11-367F23.2

3-O-C12-HSL inhibition the expression of RP11-367F23.2 dependent on PPARγin dendritic cells

目的 研究N-3-氧代十二烷酰-L-同型丝氨酸内酯(3-O-C 12 -HSL)是否抑制单核细胞来源树突状细胞(Mo-DCs)表达RP11-367F23.2,该抑制作用是否通过过氧化物酶增殖物激活受体γ(PPARγ)介导。方法 采用密度梯度离心法分离单核细胞来源的Mo-DCs;将Mo-DCs细胞分为4组:脂多糖(LPS)组、3-O-C 12 -HSL组、GW9662组和PBS组,18 h后收集细胞;荧光定量PCR检测4组中RP11-367F23.2表达情况。结果 Mo- DCs经3-O-C 12 -HSL刺激后,RP11-367F23.2表达量降低,3-O-C 12 -HSL组和LPS组RP11-367F23.2表达量比较,差异有统计学意义( P <0.05);Mo-DCs经GW9662处理后,细胞中的RP11-367F23.2表达量升高,3-O-C 12 -HSL组和GW9662组RP11-367F23.2表达量比较,差异有统计学意义( P <0.05)。结论 3-O-C 12 -HSL通过PPARγ拮抗Mo-DCs表达RP11-367F23.2。

Objective To study whether N-3-oxododecanoyl-L-homoserine lactone (3-O-C 12 -HSL) inhibits RP11-367F23.2 expression in monocytes derived-dendritic cells (Mo-DCs),and whether the inhibition is mediated by PPAR gamma. Methods Density gradient centrifugation was used to separate Mo-DCs from monocytes.Mo-DCs cells were divided into four groups:LPS group,3-O-C 12 -HSL group,PBS group,and GW9662 group.Cells were collected 18 h after treated,and real-time PCR was used to detect RP11-367F23.2 expression in four groups. Results After 3-O-C 12 -HSL treatment of Mo-DCs,the expression of RP11-367F23.2 decreased,and the expression of RP11-367F23.2 in 3-O-C 12 -HSL group and LPS group had significant difference ( P <0.05);the expression of RP11-367F23.2 in Mo-DCs cells increased after GW9662 treatment,and the expression of RP11-367F23.2 in 3-O-C 12 -HSL group and GW9662 group had significant difference ( P <0.05). Conclusion 3-O-C 12 -HSL inhibition the expression of RP11-367F23.2 dependent on PPARγ in dendritic cells.