摘要
目的 检测慢性阻塞性肺疾病(COPD)患者外周血单个核细胞(PBMCs)中微小RNA-31(miR-31)表达量并探讨其临床意义。方法 采用实时荧光定量聚合酶链反应(qPCR)的方法检测38例COPD患者(COPD组)、15例急性支气管炎(AB)患者(AB组)和40例体检健康者(对照组)PBMCs中miR-31的表达,并对15例治疗后患者进行跟踪检测。酶联免疫吸附测定法(ELISA)检测COPD患者血浆中白细胞介素4(IL-4)和γ-干扰素(IFN-γ)水平。结果 COPD 患者PBMC中miR-31的表达明显高于对照组和AB组,AB组PBMC中miR-31的表达明显高于对照组;COPD患者治疗后好转后miR-31的表达明显下降;COPD患者临床症状严重者其PBMC中miR-31的表达更高,COPD患者PBMC中miR-31的表达与血浆CRP水平呈负相关( r =-0.663 8, P <0.05),COPD患者PBMC中miR-31的表达与血浆中IL-4水平呈负相关( r =-0.476 4, P < 0.05 )。结论 COPD患者PBMC中miR-31的表达参与COPD的发生与发展,其可能在Th2类细胞中起重要作用。
Objective To investigate the expression of miR-31 in peripheral blood mononuclear cells(PBMCs) of patients with chronic obstructive pulmonary disease(COPD) and explore its clinical significance. Methods The expression of miR-31 in PBMCs from 38 patients with COPD(COPD group),15 patients with acute bronchitis(AB group),40 Healthy persons (control group) and 15 patients treated after tracking were measured by qPCR.The plasma level of IL-4 and IFN-γ in patients with COPD were measured by enzyme linked immunosorbent assay. Results The expression of miR-31 in PBMC of patients with COPD was higher than AB group and control group,the expression of miR-31 in PBMC of patients with AB group were higher than control group,and it was decreased after treatment in patients with COPD;The expression of miR-31 in serious patients with COPD was higher than the other patients,the expression of miR-31 in patients with COPD was negative correlated with CRP level( r =-0.663 8, P <0.05). The expression of miR-31 in patients with COPD was negative correlated with the level of IL-4 in plasma( r =-0.476 4, P <0.05). Conclusion The expression of miR-31 in patients with COPD is significantly increased,which is related with the occurrence and development of COPD,and it is probably to impact the function of Th2 type cells.
作者
陈锦暖
吴雷
叶树培
陈美华
CHEN Jinnuan;WU Lei;YE Shupei;CHEN Meihua(Department of Respiratory Medicine,the Third People′s Hospital ofDongguan City,Dongguan,Guangdong 523326,China)
出处
《国际检验医学杂志》
CAS
2019年第14期1737-1740,1745,共5页
International Journal of Laboratory Medicine