贵州地区紫云花猪腹泻病原菌的分离鉴定及耐药性分析

Isolation, identification and drug resistance of pathogenic diarrhoea bacteria in Ziyunhua pigs in the Guizhou area

为了鉴定贵州地区紫云花猪腹泻病原菌,分析其耐药性,试验采用细菌学常规鉴定、16SrRNA基因测序、K-B药敏纸片法对从贵州地区不同养猪场采集的患腹泻紫云花猪肝脏、肛拭子、粪便等样品196份进行细菌分离鉴定,并分析其耐药性。结果显示,从采集的196份样品中分离得到了60株大肠杆菌、35株沙门菌、20株志贺菌,分离的60株大肠杆菌对庆大霉素、阿莫西林、强力霉素等5种药物的耐药率在70.0%以上,对其他药物耐药率在15.0%~40.0%之间,耐11、10种药物的菌株最多,分别占分离菌株31.7%、23.3%;分离的35株沙门菌对氨苄西林、阿莫西林、氟苯尼考等5种药物的耐药率在58.3%以上,对其他药物耐药率在48.6%~17.1%之间,耐10、9种药物的菌株最多,分别占分离菌株25.7%、22.9%;分离的20株志贺菌对氨苄西林、阿莫西林、新霉素等9种药物的耐药率在65.0%以上,对其他药物的药物耐药率在25%~35%之间,耐9、8种药物的菌株最多,分别占分离菌株30.0%、20.0%。本研究为该地区紫云花猪细菌性腹泻的防控提供研究基础。

In order to identify the pathogenic bacteria and its drug tolerance in Ziyunhua pigs in the Guizhou area, 196 samples were collected from the livers, anal swabs and feces of the bacteria-inflicted pigs in the area. Bacteria were isolated from the samples and identified using the bacteriological routine identification, 16 S rRNAPCR and K-B drug sensitive disk methods, and were given drug resistance analysis. The results were that 60 strains of E.coli, 35 strains of Salmonella and 20 strains of Shigella were isolated. The strains of E. coli had over 70% resistance against gentamicin, amoxicillin and doxycycline, and 15.0% to 40.0% resistance against the other drugs. Most of the strains resisted against 11 or 10 kinds of drugs, accounting for 31.7% and 23.3% of the isolated strains, respectively. The Salmonella isolates had over 58.3% resistance against ampicillin, amoxicillin and florfenicol and 48.6% to 17.1% resistance against the other drugs. Most of the strains resisted against 10 and 9 drugs, accounting for 25.7% and 22.9% of the isolates, respectively. The strains of Shigella had over 65.0% resistance aginst ampicillin, amoxicillin and neomycin, and 25% to 35% resistance against the other drugs. Most og the strains resisted against 9 and 8 drugs, accounting for 30.0% and 20.0% of the isolated strains, respectively. This study provided a basis for the prevention and control of bacterial diarrhea in Ziyunhua pigs in the Guizhou area.