梅毒检验中酶联免疫吸附法与甲苯胺红不加热血清法的比较

Comparison of Enzyme-Linked Immunosorbent Assay and Toluidine Red Unheated Serum Assay in Syphilis Detection

为了分析比较甲苯胺红不加热血清法与酶联免疫吸附法对梅毒诊断的区别,并找到2种方法在梅毒诊断中的优势,选取了汕头市中心医院2018年3月—2019年5月性病门诊收治的病患200例作为研究对象,随机分为实验组和对照组。实验组100例,采用甲苯胺红不加热血清法对患者进行梅毒螺旋体抗体检测;对照组100例,采用酶联免疫吸附法对研究对象进行梅毒螺旋体抗体检测。结果显示,酶联免疫吸附法检测血清阳性率明显高于甲苯胺红不加热血清法,差异有统计学意义(P<0.05);酶联免疫吸附法检测结果的敏感性、阳性预测值均高于甲苯胺红不加热血清法,差异有统计学意义(P<0.05)。由此得出,酶联免疫吸附法在梅毒检验中阳性率较高,值得临床推广应用。

In order to analyze and compare the difference between toluidine red unheated serum method and enzyme-linked immunosorbent assay(ELISA)in the diagnosis of syphilis,and to find the advantages of two methods in the diagnosis of syphilis,200 patients admitted to STD clinic of Shantou Central Hospital from March 2018 to May 2019 are randomly divided into experimental group and control group,100 patients in experimental group are treated with toluidine red unheated serum method.Treponema pallidum antibody detection;control group 100 cases,using enzyme-linked immunosorbent assay for Treponema pallidum antibody detection.The results show that the positive rate of serum detected by ELISA is significantly higher than that of non-heated serum detected by toluidine red and has significant difference(P<0.05),and the sensitivity and positive predictive value of ELISA are higher than that of non-heated serum detected by toluidine red and has significant difference(P<0.05).It is concluded that the positive rate of ELISA in syphilis test is higher,which is worthy of clinical application.