外源激素对洒金柏离体培养的影响

Effect of External Hormones in Vitro Culture of Sabina chinensis(L.)Ant.cv.Aurea

[目的]研究不同外援激素对洒金柏离体培养的影响,初步筛选出芽萌发、丛生芽及生根诱导的培养基配方。[方法]试验以洒金柏(Sambucus canadensis L.)嫩枝茎段为外植体,研究不同激素配比对丛生芽诱导的影响。[结果]洒金柏的最适芽萌发培养基为1/2MS+6-BA(0.2mg/L)+NAA(0.1mg/L),萌发率96%;丛生芽诱导最佳增殖培养基为1/2MS+6-BA(0.5mg/L)+NAA(0.1mg/L),增殖系数为6.8;最适生根培养基为1/2MS+IBA(0.5mg/L)+NAA(0.2mg/L),生根率93%。[结论]不同的外源激素有效提高洒金柏的离体培养效率,为其快速繁殖提供理论基础。

[Objection]The effects of different foreign hormones on the off-topic culture of Sambucus chinensis were studied.And the medium formulations for bud germination and root induction were screened out.[Method]Taking stem as explants,the effect of bud induction was studied with different hormone combination.[Result]The best suitable bud enrichment medium was 1/2MS+6-BA 0.2 mg/L+NAA 0.1 mg/L.The germination rate was 96%,and the plants growed healthy,normal,flat leaves internodes.The best proliferation hormone ratio was 1/2MS+6-BA 0.5 mg/L+NAA 0.1 mg/L.The best and the proliferation coefficient reached 6.8.The optimum root inducing medium was 1/2MS+IBA 0.5 mg/L+NAA 0.2 mg/L,and rooting rate reached 93%.[Conclusion]The research improved the efficiency of in vitro culture of Sambucus chinensis effectively and provided theoretical basis for its rapid propagation.