摘要
[目的]研究不同外援激素对洒金柏离体培养的影响,初步筛选出芽萌发、丛生芽及生根诱导的培养基配方。[方法]试验以洒金柏(Sambucus canadensis L.)嫩枝茎段为外植体,研究不同激素配比对丛生芽诱导的影响。[结果]洒金柏的最适芽萌发培养基为1/2MS+6-BA(0.2mg/L)+NAA(0.1mg/L),萌发率96%;丛生芽诱导最佳增殖培养基为1/2MS+6-BA(0.5mg/L)+NAA(0.1mg/L),增殖系数为6.8;最适生根培养基为1/2MS+IBA(0.5mg/L)+NAA(0.2mg/L),生根率93%。[结论]不同的外源激素有效提高洒金柏的离体培养效率,为其快速繁殖提供理论基础。
[Objection]The effects of different foreign hormones on the off-topic culture of Sambucus chinensis were studied.And the medium formulations for bud germination and root induction were screened out.[Method]Taking stem as explants,the effect of bud induction was studied with different hormone combination.[Result]The best suitable bud enrichment medium was 1/2MS+6-BA 0.2 mg/L+NAA 0.1 mg/L.The germination rate was 96%,and the plants growed healthy,normal,flat leaves internodes.The best proliferation hormone ratio was 1/2MS+6-BA 0.5 mg/L+NAA 0.1 mg/L.The best and the proliferation coefficient reached 6.8.The optimum root inducing medium was 1/2MS+IBA 0.5 mg/L+NAA 0.2 mg/L,and rooting rate reached 93%.[Conclusion]The research improved the efficiency of in vitro culture of Sambucus chinensis effectively and provided theoretical basis for its rapid propagation.
作者
陈雪
孙丽娜
王媚
安冬
王淼
商超
吴春华
CHEN Xue(Dalian Modern Agricultural Development Service Center,Dalian,Liaoning 116030)
出处
《园艺与种苗》
CAS
2019年第6期24-26,32,共4页
Horticulture & Seed
关键词
洒金柏
组织培养
快繁
Sabina chinensis(L.)Ant.cv.Aurea.
Tissue culture
Micropropagation