乌鲁木齐地区孕妇B族链球菌荧光PCR检测结果分析

Detection of B streptococcus in pregnant women in Urumqi area by fluorescence PCR

目的调查乌鲁木齐地区孕妇B族链球菌带菌率及带菌高危因素,分析该菌对妊娠结局的影响。方法选取2016年1月至2018年8月在乌鲁木齐市妇幼保健院常规产检的孕妇作为研究对象,取阴道和肛门分泌物,采用荧光聚合酶链反应(PCR)检测B族链球菌,统计孕妇B族链球菌带菌率。将孕妇按不同民族、年龄、孕周、是否多次妊娠、甲状腺功能减退、妊娠期糖尿病、瘢痕子宫等临床资料分组,分析孕妇妊娠期感染B族链球菌的高危因素,并比较B族链球菌阳性组与B族链球菌阴性组早产和胎膜早破发生率是否有显著性差异。结果乌鲁木齐地区孕妇B族链球菌带菌率为4.05%,带菌率略低于国内其他地区;logistic回归分析显示:B族链球菌带菌状况与孕妇年龄、甲状腺功能减退、妊娠期糖尿病显著相关(P<0.05);χ2检验提示:B族链球菌阳性组与阴性组早产、胎膜早破发生率比较,差异均无统计学意义(χ2=1.852、2.160,P=0.167、0.160)。结论高龄、妊娠合并甲状腺功能减退、妊娠期糖尿病会增加B族链球菌感染风险,提示产科医生在预防产褥期感染中需要格外关注此类孕妇。

Objective To investigate the carrier condition and high risk factors of group B streptococcus(GBS)in the pregnant women in Urumqi,and analyze the effect of the bacteria on pregnancy outcome.Methods The genital tract secretion samples from pregnant women were studied from January 2016 to August 2018 in Urumqi.GBS carrier rate in Urumqi was detected by Fluorescent quantitative polymerase chain reaction(PCR).The clinical data was divided to different groups by different nationality,age,gestational age,whether with multiple pregnancy,hypothyroidism,gestational diabetes,and the high risk factors of GBS infection were analyzed.The pregnant outcomes were compared between GBS positive group and GBS negative group by statistics.Results The result showed that the GBS carrier rate was 4.05%in Urumqi.Logistic regression analysis showed that the carrier status of GBS was significantly correlated with the age of pregnant women,hypothyroidism and gestational diabetes mellitus(P<0.05);χ2 test indicated that there was no statistically significant difference in the incidence of premature labor and premature rupture of membranes between GBS positive group and negative group(χ2=1.852,2.160,P=0.167,0.160)Conclusion Older age,pregnancy with hypothyroidism and gestational diabetes mellitus increase the risk of GBS infection.There are not significant differences between GBS positive group and GBS negative group in incidence of preterm birth and premature rupture of membranes.