牦牛血抗氧化低聚肽制备工艺的优化及抗缺氧活性的初探

Optimization of preparation process of yak blood antioxidant oligopeptides and preliminary study of anti-hypoxia activity

为简化牦牛血抗氧化低聚肽的制备工艺并对其抗缺氧活性进行初探,实验采用菌酶联合发酵、超滤分级制备牦牛血抗氧化低聚肽,常压耐缺氧实验以及亚硝酸钠中毒存活实验评价其抗缺氧活性。结果表明:1kDa超滤分级得到的牦牛血抗氧化低聚肽具有良好的体外抗氧化活性,·OH清除能力、DPPH·清除能力、总抗氧化力以及脂质过氧化抑制能力均极显著优于商品化大豆低聚肽(P<0.01);不同剂量的牦牛血抗氧化低聚肽对小鼠常压缺氧存活时间以及亚硝酸钠中毒存活时间均有不同程度的延长,并呈现剂量依赖性。综上,牦牛血抗氧化低聚肽可作为一种天然抗氧化剂,且具有提高小鼠抗缺氧应激的能力。

In order to simplify the preparation process of yak blood antioxidant oligopeptides and study their antihypoxic activity,yak blood antioxidant oligopeptides were prepared by bacteria enzyme combined fermentation,5 kDa and 1 kDa ultrafiltration classification,the anti-hypoxia activities were evaluated by atmospheric hypoxia tolerance test and sodium nitrite poisoning survival test.The results showed that yak blood antioxidant oligopeptides had good antioxidant activity in vitro,·OH scavenging ability,DPPH·scavenging activity,total antioxidant ability,total reducing power and lipid peroxidation inhibition were significantly better than commercial soybean oligopeptides in vitro(P<0.01);different doses of yak blood antioxidant oligopeptides prolonged the survival time of mice under normal pressure hypoxia and sodium nitrite poisoning to varying degrees,and dose-dependent.In conclusion,yak blood antioxidant oligopeptides could be used as a natural antioxidant,and could improve the ability of mice to resist hypoxic stress,and the mechanism of anti-hypoxia could be further studied.