基于功能化还原氧化石墨烯和金钯合金纳米粒子的谷氨酸电致化学发光生物传感器研究

A stereoselective electrochemiluminescence biosensor based on hemin functionalized reduced graphene oxide nanosheets and Au-Pd alloy nanoparticles

该实验设计了基于氯化血红素功能化的还原氧化石墨烯(H-RGO)、金钯合金纳米粒子(Au-PdNPs)和L-谷氨酸氧化酶(L-GluOx)的特异性L-谷氨酸(L-Glu)电致化学发光(ECL)生物传感器。实验首先将H-RGO修饰于电极上,再通过电沉积的方式将Au-PdNPs负载于H-RGO表面,L-GluOx通过与Au-PdNPs的键合作用实现固载,构建ECL生物传感界面。纳米材料的制备以及传感界面的逐步构建过程通过扫描电子显微镜(SEM)、X-射线能量散射谱(EDS)、紫外可见吸收光谱技术(UV-vis)和电化学技术等测试方法进行了验证。实验发现纳米材料Au-PdNPs和H-RGO二者的协同催化作用极大的改进了传感器的灵敏度。在优化的条件下,L-谷氨酸在1.0×10^-7mol/L至5.00×10^-3mol/L浓度范围内,ECL信号强度与其浓度对数呈线性相关性。该ECL生物传感器制备简单、响应快速、检测灵敏、稳定性和选择性好,在谷氨酸的检测应用方面具有较大的发展潜力。

This paper reported a stereoselective electrochemiluminescence (ECL) biosensor based on hemin functionalized reduced graphene oxide nanosheets (H-RGO), Au-Pd alloy nanoparticles (Au-PdNPs) and L-glutamate oxidase (L-GluOx) for the chiral recognition of L-glutamate (L-Glu). It was relatively simple that Au-PdNPs were covered on the H-RGO modified glass carbon electrodes via electrodeposition. The synergistic catalytic effect between H-RGO and Au-PdNPs improved the analytical performances of the ECL biosensor. Amount of L-GluOx can be immobilized on the electrode. The stepwise fabrication of nanomaterials was characterised by scanning electron microscopy (SEM), X-ray energy-dispersive spectroscopy (EDS), UV-vis spectra and electrochemical methods. Un-derthe optimized conditions, the ECL biosensor showed good analytical performances in detection of L-Glu with a wide linear range from 1.0×10^-7 to 5.0×10^-3 mol/L, good stability and excellent selectivity. Therefore, this strategy would provide a new idea for selective detecting other targets based on the same principle.