除膻菌株的功能性分析

Functional Analysis of Degrading Astringence Strains

将4株具有除膻效果的霉菌进行功能性分析、包括脂肪酶活力测定试验、生长曲线的测定、产H2S和产生物胺测定试验、抑菌试验、耐盐性和耐亚硝酸盐性试验、拮抗试验与复配试验。结果表明,菌株THZ1、TZH3、TZH4和4QT3的脂肪酶活力分别为4.35、2.34、3.62、2.63 U/mL。4株菌均不产H2S,菌株4QT3产生物胺,其余3株菌不产生物胺。菌株TZH3和TZH4的抑菌能力、耐盐性和耐亚硝酸盐性较好。最终选择TZH3和TZH4进行拮抗试验及复配试验。结果显示,菌株TZH3和THZ4不具有拮抗作用,当TZH3和TZH4的复配体积比为1 ∶ 1时,生长情况较优。

Functional analysis of 4 molds with degrading astringence was carried out, including lipase activity test, growth curve test, H2S and biogenic amine production test, bacteriostatic test, salt and nitrite tolerance test, antagonist test and compound test. The results showed that the lipase activities of THZ1, TZH3, TZH4 and 4QT3 were 4.35, 2.34, 3.62 U/mL and 2.63 U/mL. None of the four strains produced H2S, strain 4QT3 produced amines and the other 3 strains did not produce amines. TZH3 and TZH4 have better antibacterial, salt and nitrite tolerance. Finally, TZH3 and TZH4 were selected for antagonistic test and compound test. The results showed that TZH3 and THZ4 did not have antagonistic effects. When the compounding ratio of TZH3 and TZH4 was 1 ∶ 1, the growth was better.