紫草素对缺血性脑损伤大鼠脑组织保护作用的研究

Protective Effect of Shikonin on Brain Tissues of Rats with Ischemic Brain Injury

目的 探讨紫草素对缺血性脑损伤大鼠脑组织的保护作用及其机制。方法 选取48只大鼠,随机分为假手术组、模型组和紫草素治疗组,每组16只。制备缺血性脑损伤大鼠模型,观察3组大鼠日常行为,并对大鼠脑组织进行病理学观察,用酶联免疫吸附试验测定3组脑组织中白介素-6(IL-6)和IL-8含量,蛋白免疫印迹法检测3组脑核因子-κB(NF-κB)蛋白表达水平。结果 模型组动物活动减少,部分动物出现躁动。假手术组大鼠皮层及海马区锥体神经元层次分明;模型组皮层及海马区神经细胞层次紊乱,密度降低;紫草素治疗组可以改善缺血性脑损伤大鼠脑组织神经元的异常形态变化。假手术组神经元形态基本正常;模型组神经元细胞胞质内空泡形成,细胞核内染色质边集;紫草素治疗组神经元细胞状态有所好转,细胞变性程度较模型组缓解。紫草素治疗组IL-6和IL-8表达量及NF-κB蛋白表达水平均低于模型组(P<0.01)。结论 紫草素对缺血性脑损伤大鼠有一定改善作用。

Objective To investigate protective effect and mechanism of Shikonin on brain tissues of rats ischemic brain injury. Methods A total of 48 rats were randomly divided into sham operation group, model group and Shikonin treatment group ( n =16 in each group). Rats models with ischemic brain injury were prepared to observe daily behavior, and pathological observation for brain tissues were performed. Enzyme linked immunosorbent assay was used to detect contents of interleukin-6 (IL-6) and interleukin-8 (IL-8), and protein expression levels of nuclear factor-κB (NF-κB) were detected by Western blot among three groups. Results In model group, animal activity was decreased, and some animals appeared restlessness;In sham operation group, neurons in cortex and hippocampal cone ordered clearly. In model group, neurons cell layers in cortex and hippocampal cone disordered with decreased density. Shikonin treatment group could improve abnormal morphological changes of brain tissues in rats with ischemic brain injury. Neurons appearances were basically normal in sham operation group. Cytoplasmic vacuolation and endonuclear chromatin margination were observed in model group. In Shikonin treatment group, conditions of neurons cells were improved, and degree of cell degeneration relieved than that in model group. Expression of IL-6 and IL-8 and NF-κB protein expression levels in Shikonin treatment group were lower than those in model group ( P <0.01). Conclusion Shikonin has a certain effect in improvement of brain injuries in rats with ischemic brain injury.