两色金鸡菊总黄酮部位多指标成分含量测定

Multiple Components Determination of Flavonoids Fraction of Coreopsis tinctoria Nutt.

目的:采用超高效液相色谱-二极管阵列检测器(UPLC-DAD)建立两色金鸡菊Coreopsis tinctoria Nutt.中R/S-异奥卡宁-7-O-β-D-葡萄糖苷、对羟基桂皮酸甲酯-4-O-β-D-吡喃葡萄糖苷、槲皮万寿菊素-7-O-β-D-葡萄糖苷、异奥卡宁、槲皮素-7-O-葡萄糖苷、马里苷、金鸡菊苷、奥卡宁、圣草酚、紫铆花素10种成分含量测定方法,为两色金鸡菊药理研究及药效物质基础研究提供参考。方法:采用Waters ACQUITY UPLC HSS T3色谱柱(100mm×2.1mm,1.8μm);流动相为0.1%甲酸水和乙腈;流速为0.3mL·min^-1;柱温为30℃;紫外检测波长为285nm与378nm;进样量为3μL。结果:建立两色金鸡菊总黄酮部位超高效液相方法,方法学考察结果符合《中华人民共和国药典》相关要求,测定了10种成分含量。结论:建立的两色金鸡菊总黄酮部位10种化学成分含量测定方法稳定、重复性好、精密度高,可为两色金鸡菊总黄酮部位的质量控制及药理研究提供参考。

Objective:To establish a UPLC-DAD method for content determination of ( R/S )-flavanomarein,linocinnamarin,quercetagetin-7- O-β-D-glucoside,isookanin,quercetin-7- O-glucoside,marein,butein-4- O-β-D-glucopyranoside,okanin,eriodictyol and butein from Coreopsis tinctoria flavonoids fraction and provide a reference for the pharmacological research and of C.tinctoria. Method:Waters ACQUITY UPLC HSS T3 column (100 mm×2.1 mm,1.8 μm )was applied with mobile phase of 0.1% formic acid and acetonitrile.The flow rate was 0.3 mL · min^-1 and column temperature was 30 ℃,and UV detection wavelength was 285 nm and 378 nm. Results:The UPLC method for the flavonoids fraction of C.tinctoria was established.The methodological study conformed to the requirements of Pharmacopoeia of People′s Republic of China. Conclusion :The method for content determination of 10 chemical constituents in the flavonoids fractions of C.tinctoria was verified to be stable,reproducible and high-precision,which can provide a basis for quality control and pharmacological research of the flavonoids fractions of C.tinctoria.