衰老小鼠肝脏、脾脏和骨髓中Blm解旋酶表达水平

Changes of Bloom’s Syndrome Helicase in Liver,Spleen and Bone Marrow of Senile Mice

目的:观察Blm解旋酶在衰老小鼠肝、脾及骨髓组织中的表达水平。方法:20只小鼠均分为正常对照组和衰老组,雌雄各半,采用D-gal致衰老的方法制备衰老小鼠模型;观察小鼠生存状态、监测体质量,检测小鼠血清中超氧化物气化酶(SOD)、丙二醛(MDA)含量和小鼠肝脏中凋亡相关蛋白Bax的表达量;利用蛋白质印记法和石蜡切片免疫组织化学染色方法检测小鼠肝脏、脾脏和骨髓中Blm蛋白的表达。结果:衰老模型组小鼠生存状态变差,体质量增长低于对照组小鼠;与对照组小鼠比较,血清SOD活性降低、MAD含量升高、Bax蛋白表达增多,差异有统计学意义(P<0.05);衰老模型组小鼠的脾脏H-E切片边缘区模糊,白髓与红髓界限模糊;胸腺H-E切片显示衰老模型组小鼠胸腺皮质、髓质界限模糊、胸腺细胞分布稀疏;衰老组小鼠Blm解旋酶在肝脏、脾脏和骨髓的表达均低于对照组小鼠,差异有统计学意义(P<0.05)。结论:Blm解旋酶在衰老小鼠体内的表达降低,Blm解旋酶可能与衰老发生的机制相关。

Objective:To explore the changes of the expression of Bloom's syndrome helicase(BLM)in senile mice.Method:C57BL/6 mice were injected with D-galactose.Their state of life and biological behaviors were observed everyday.SOD activities and MDA content of serum were measured,and the expression of Bax protein in the liver and the changes of the structure of spleen and thymus were observed by H-E staining method to determine whether the senile mouse models were successfully established.The expressions of BLM protein in the liver,spleen and bone marrow of the successfully established models were observed by Western blotting and immunohistochemistry.Results:The model mice showed inferior state of life and constitution compared with those of the normal mice.The activities of SOD in the serum decreased significantly and the content of MDA in the serum increased significantly(P<0.05).Bax in the liver increased.H-E spleen section of model mice indicated that the boundary between white pulp and red pulp became unclear and the marginal zone was blurred.H-E thymic section indicated that the boundary between cortex and medulla became unclear,and the Thymic cells were sparse.The D-galactose subacute senile mouse models were successfully established.BLM protein in liver,spleen and bone marrow decreased in senile mice(P<0.05).Conclusion:BLM protein in senile mice decreased,and the mechanism may be related to the occurrence of senility.