大气压等离子体射流对PLLA纤维膜表面改性方法的研究

Research on Surface Modification of PLLA Fibrous Membranes with Atmospheric Pressure Plasma Jet

左旋聚乳酸(PLLA)以其优异的生物可降解性能和良好的力学性能在组织工程中得到了广泛的应用。其中,PLLA纤维膜多孔结构模拟了天然细胞外基质,因而引起了广泛关注。然而,PLLA纤维膜的疏水性能使其细胞亲和力较差,进而限制了它与生物环境的相互作用。大气压等离子体射流(APPJ)是一种有效的表面改性工具,具有结构简单、成本低和操作灵活等优点。研究使用静电纺丝法成功制备了平均纤维直径为1 184nm的PLLA纤维膜,并通过He/O2 APPJ对PLLA纤维膜进行表面改性,大幅提高了材料表面的亲水性和细胞亲和力。在改性前后的PLLA纤维膜上培养小鼠成肌细胞C2C12,结果表明,改性后的PLLA纤维膜更有利于细胞的增殖和生长。研究结果在组织工程和生物医学工程中具有重要的应用前景。

Poly(L-Lactic)Acid(PLLA)was widely used for tissue engineering due to its excellent biodegradability and good mechanical properties.Among them,the porous structure of the PLLA fibrous membrane simulated the natural extracellular matrix,thus it attracted much attention.However,the hydrophobic nature of the PLLA fibrous membrane resulted in poor cell affinity,which in turn limited its interaction with the biological environment.The atmospheric pressure plasma jet(APPJ)was an effective surface modification tool with the advantages of simple structure,low cost and flexible operation.The PLLA fibrous membrane with an average fiber diameter of 1184 nm was successfully prepared by electrospinning,and the surface modification of PLLA fibrous membrane by He/O2 APPJ greatly improved the hydrophilicity and cell affinity of the material surface.Mouse myoblast C2 C12 was cultured on the PLLA fibrous membrane before and after modification,the results showed that the modified PLLA fibrous membrane was more conducive to cell proliferation and growth.The research results had important application prospects in tissue engineering and biomedical engineering.