摘要
建立高效液相色谱法同时测定木姜叶柯中三叶苷、根皮苷和根皮素的含量。采用C18色谱柱;检测波长285nm,流动相为乙腈-0.04%甲酸,梯度洗脱;流速0.8mL/min;柱温40℃。三叶苷、根皮苷和根皮素的加标回收率分别为102.54%、102.01%和102.12%,RSD分别为1.66%、2.21%和2.38%。该方法适用于木姜叶柯中根皮苷、三叶苷和根皮素的含量分析,为其药材质量的判定提供数据参考。
High performance liquid chromatography(HPLC)was used to determine phlorizin,trilobatin and phloretin in Lithocarpus litseifolius.The separation of target components was performed on a C18 column.The detection wavelength was 285 nm,the mobile phase was acetonitrile-water(0.04%formic acid),with gradient elution.The flow rate was 0.8 mL/min.Column temperature was 40℃.The standard recoveries of phlorizin,trilobatin and phloretin were 102.54%(RSD 1.66%),102.01%(RSD 2.21%)and 102.12%(RSD 2.38%),respectively.This method can be used to analyze the content of phlorizin,trilobatin and phloretin simultaneously,and can provide reference for quality control of the L.litseifolius.
作者
王钰
王磊
张东星
李赫宇
晏仁义
WANG Yu;WANG Lei;ZHANG Dongxing;LI Heyu;YAN Renyi(Tianjin Ubasio Biotechnology Group Co.,Ltd.,Tianjin 300457,China)
出处
《天津科技》
2019年第7期66-68,70,共4页
Tianjin Science & Technology