辣木叶乙醇提取物对非酒精性脂肪肝小鼠模型血脂和氧化应激影响的研究

Effects of ethanol extract of Moringa oleifera leaves on oxidative stress and lipid in nonalcoholic fatty liver disease mice model

该研究以低剂量(5mg·kg^-1)、中剂量(30mg·kg^-1)和高剂量(60mg·kg^-1)的辣木叶乙醇提取物(EE-MO)干预高脂饮食诱导的非酒精性脂肪肝(NAFLD)小鼠动物模型。结果表明:(1)高剂量的EE-MO显著降低NAFLD小鼠的体重和肝湿重;EE-MO剂量依赖性地降低NAFLD小鼠血清TC、TG、HDL-C和LDL-C含量;高剂量的EE-MO除降低上述生化指标外,还显著降低血清中FFA含量。(2)HE和苏丹红Ⅲ染色发现,EE-MO处理后,模型组小鼠的肝脂肪病变和细胞损伤得到显著改善。(3)EE-MO对NAFLD小鼠模型的血脂代谢具有改善作用。(4)高脂饮食诱导小鼠肝脏和血清的ROS和MDA的含量,诱导SOD、POD和CAT活性增加,降低GSH-Px活性。(5)低剂量、中剂量和高剂量的EE-MO依赖性地降低NAFLD小鼠肝脏和血清的ROS和MDA的含量,缓解氧化胁迫。(6)低剂量的EE-MO对SOD、POD、CAT和GSH-Px酶活性无显著影响;中剂量和高剂量的EE-MO处理后,NAFLD小鼠的SOD、POD和CAT酶活性显著下降,GSH-Px活性显著增加;EE-MO可能通过GSH-Px抗氧化酶途径缓解NAFLD小鼠的氧化胁迫。

Lower (5 mg·kg^-1 ), middle (30 mg·kg^-1 ) and high (60 mg·kg^-1 ) doses of ethanol extract of Moringa oleifera (EE-MO) leaves was used to interpose the high fat diet-induced non-alcoholic fatty liver disease (NAFLD) mice model.The results were as follows:(1) That oral administration of high dose EE-MO could significantly reduce body weight and wet liver weight of NAFLD mice;EE-MO treatment could decrease the TC, TG, HDL-C and LDL-C contents in serum of NAFLD mice in dose-dependant manner;Apart from decreasing the above parameters, high dose EE-MO treatment significantly reduced FFA content in serum of NAFLD mice.(2) HE and Sudan red Ⅲ staining showed that the fatty lesion and hepatocyte injury of NAFLD mice were improved by EE-MO treatment.(3) It was suggested that EE-MO treatment was capable of improving lipid metabolism of NAFLD mice.(4) High fat diet increased the contents of liver and serum ROS and MDA as well as the activities of SOD, POD and CAT.However, the GSH-Px activity was suppressed by high fat diet.(5) EE-MO treatment could decrease the ROS and MDA contents in liver and serum of NAFLD mice in dose-dependant manner.(6) Oral administration of low dose EE-MO exerted no effect on the activities of SOD, POD, CAT, and GSH-Px of NAFLD mice;However, oral administration of middle and high dose EE-MO significantly reduced the activities of SOD, POD and CAT, but increased the GSH-Px activity;It isuggested that the EE-MO treatment can alleviate the oxidative stress of NAFLD mice by regulating GSH-Px activity.