lncRNA UCA1通过抑制miRNA-203a对乳腺癌细胞化疗耐药作用的影响

Effect of IncRNA UCA1 on chemotherapy resistance of breast cancer via miRNA-203a inhibition

目的探讨长链非编码RNA(lncRNA)尿路上皮癌相关1(UCA1)基因通过抑制微小RNA-203a(miR-NA-203a)对乳腺癌细胞化疗耐药性的影响.方法选取MCF7、MCF7/5-FU细胞,采用UCA1 siRNA对两种细胞进行转染,分为MCF7干扰组、MCF7未干扰组和MCF7/5-FU干扰组、MCF7/5-FU未干扰组;采用不同浓度奥沙利铂处理干扰后MCF7细胞,取最佳抑制浓度的奥沙利铂处理干扰后MCF7、MCF7/5-FU细胞,采用实时定量聚合酶链反应(RT-PCR)检测MCF7、MCF7/5-FU细胞中lncRNA UCA1的表达情况及干扰后MCF7、MCF7/5-FU细胞中miRNA-203a的表达水平,噻唑蓝(MTT)法检测干扰后MCF7、MCF7/5-FU细胞的活力.结果 MCF7/5-FU细胞中lncRNA UCA1的相对表达量明显高于MCF7细胞(P﹤0.01);MCF7干扰组MCF7细胞的存活率明显低于MCF7未干扰组,miRNA-203a的相对表达量明显高于MCF7未干扰组(P﹤0.01);MCF7/5-FU干扰组MCF7/5-FU细胞的存活率明显低于MCF7/5-FU未干扰组,miRNA-203a的相对表达量明显高于MCF7/5-FU未干扰组(P﹤0.01).结论 LncRNA UCA1可通过抑制miRNA-203a表达增强乳腺癌细胞的化疗耐药性,增加乳腺癌细胞的活力.

Objective To investigate the effect on chemotherapy resistance by long noncoding RNA (lncRNA) urothelial cancer associated 1 (UCA1) via micro RNA-203a (miRNA-203a) inhibition. Method MCF7 and MCF7/5-FU cells were selected, and were transfected with UCA1 siRNA. The cells were then divided into MCF7 interference group, MCF7 non- interference group, MCF7/5-FU interference group and MCF7/5-FU non- interference group, respectively. MCF7 cells were treated with different concentrations of oxaliplatin and optimal inhibitory concentration was found. After interference, MCF7 and MCF7/5-FU cells were treated with the optimal inhibitory concentration of oxaliplatin. Realtime quantitative polymerase chain reaction (RT-PCR) was used to detected the expression levels of lncRNA UCA1 in MCF7 and MCF7/5-FU cells, and the expression levels of miRNA-203a in MCF7 and MCF7/5-FU cells after interference. The cell viability was detected in MCF7 and MCF7/5-FU cells after interference undergoing treatment by MTT assay. Result The relative expression level of lncRNA UCA1 in MCF7/5-FU cells was significantly higher than that in MCF7 cells (P<0.01). The survival rate of MCF7 cells in MCF7 interference group was significantly lower than that in MCF7 non- interference group, and the relative expression level of miRNA-203a was significantly higher than that in MCF7 non-interference group (P<0.01). The survival rate in MCF7/5-FU interference group was significantly lower than that in MCF7/5-FU non- interference group, and the relative expression level of miRNA-203a was significantly higher than that in MCF7/5-FU non-interference group (P<0.01). Conclusion LncRNA UCA1 can enhance the chemotherapy resistance and promote the viability of breast cancer cells via miRNA-203a expression inhibition.