摘要
We designed a lysosome-selective Raman probe by conjugating bisphenylbutadiyne with morpholine, a well-known lysosome targeting moiety. This probe, named Lyso-BADY, has a Raman peak 28 times more intense than that of 5-ethynyl-2'-deoxyuridine. Lysosome in living cells was successfully visualized by hyperspectral stimulated Raman scattering (SRS) microscopy with this extracellular probe. Further study showed that the Raman signal of Lyso-BADY remained steady and strong even after a prolonged irradiation time. The photo-stability feature of Lyso-BADY rendered molecules of the similar structure as potentially versatile probe for continuous imaging in the future.
We designed a lysosome-selective Raman probe by co njugating bisphe nylbutadiyne with mo rp holine,a well-known lysosome targeting moiety.This probe,named Lyso-BADY,has a Raman peak 28 times more intense than that of 5-ethynyl-2’-deoxyuridine.Lysosome in living cells was successfully visualized by hyperspectral stimulated Raman scattering(SRS)microscopy with this extracellular probe.Further study showed that the Raman signal of Lyso-BADY remained steady and strong even after a prolonged irradiation time.The photo-stability feature of Lyso-BADY rendered molecules of the similar structure as potentially versatile probe for continuous imaging in the future
基金
the National Natural Science Foundation of China (Nos. 21432008, 91753201 and 21721005) for financial support
