超高效液相色谱测定化妆品中11种植物美白成分

Determination of 11 kinds of plant whitening ingredients in cosmetics by ultra performance liquid chromatography

采用超高效液相色谱法测定不同种类化妆品中11种植物美白成分的含量。样品用体积分数为50%甲醇提取,经AgilentEclipse色谱柱(100mm×2.1mm,1.8μm)分离,流动相采用甲醇-体积分数0.1%磷酸水溶液梯度洗脱,280和237nm波长同时检测,柱温30℃。结果表明,11种植物美白成分在进样范围内线性关系良好,相关系数均大于0.999;水、面膜、乳3种不同基质中的平均加样回收率分别为98.57%~103.44%,95.09%~112.43%和92.90%~104.21%,RSD分别为0.42%~2.96%,0.98%~2.21%和0.79%~2.67%。结果表明方法具有较高的重现性和耐用性,可用于同时检测化妆品中11种植物美白成分。

UPLC method was established for determination of 11 kinds of plant whitening ingredients in cosmetics.Samples were extracted by 50% methanol, then separated by Agilent Eclipse column(100 mm×2.1 mm, 1.8 μm)using methanol and the aqueous solution of 0.1% phosphoric acid as mobile phase with gradient elution. The detection wavelengths were 280 and 237 nm, and the column temperature was 30 ℃. All of the calibration curves show good linear relationship in the range of injection volume and all the correlation coefficients are above 0.999;the average recoveries of astringent, milk and facial mask are in the range of 98.57%~103.44%, 95.09%~112.43%and 92.90%~104.21% respectively, with RSD of 0.42%~2.96%, 0.98%~2.21% and 0.79%~2.67% respectively.The method shows good reproducibility and durability. It can be used for simultaneous determination of 11 kinds of plant whitening ingredients in cosmetics.