丘脑室旁核介导小鼠orexin能神经元的全麻促觉醒效应:采用光遗传学方法评价

Thalamic paraventricular nucleus mediates orexinergic neurons-induced promotion of emergence from general anesthesia in mice:evaluation using optogenetics method

目的采用光遗传学方法评价丘脑室旁核(PVT)介导小鼠促食欲素(orexin)能神经元的全麻促觉醒效应.方法健康雄性Hcrt-cre转基因小鼠20只,8~10周龄,体重20~25g,采用随机数字表法分为4组(n=5):逆行病毒光敏感通道组(R组)、顺行病毒光敏感通道组(A组)、逆行病毒对照组(RC组)和顺行病毒对照组(AC组).利用光遗传学技术进行处理.采用1.0%异氟醚与纯氧1.0L∕min诱导并维持麻醉,采用PowerLab脑电监测系统进行全程脑电监测,记录光刺激前1min和光刺激期间的爆发性抑制率(BSR).结果与RC组或光刺激前1min比较,R组光刺激期间BSR降低(P<0.05).与AC组或光刺激前1min比较,A组光刺激期间BSR降低(P<0.05).结论采用光遗传学技术再次证实了orexin能神经元可通过PVT发挥全麻促觉醒效应.

Objective To evaluate whether the thalamic paraventricular nucleus mediates orexinergic(orexin)neurons-induced promotion of emergence from general anesthesia by using the optogenetics method in mice.Methods Twenty healthy male Hcrt-cre mice,aged 8-10 weeks,weighing 20-25 g,were divided into 4 groups(n=5 each)using a random number table method:retrograde labeled viruses channelrhodopsin group(R group),anterograde labeled viruses channelrhodopsin group(A group),retrograde labeled viruses control group(RC group),and anterograde labeled viruses control group(AC group).The optogenetics technique was used in each group.Anesthesia was induced and maintained through inhaling 1%isoflurane and pure oxygen 1.0 L/min.Electroencephalogram was monitored througout the procedure with the PowerLab monitoring system.The burst suppression ratio(BSR)was recorded at 1 min before light stimulation and during light stimulation.Results Compared with RC group or the baseline at 1 min before light stimulation,the BSR was significantly decreased during light stimulation in R group(P<0.05).Compared with AC group or the baseline at 1 min before light stimulation,the BSR was significantly decreased during light stimulation in group A(P<0.05).Conclusion Optogenetics technique application once again confirms that orexin neurons can promote emergence from general anesthesia through thalamic paraventricular nucleus in mice.