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2017-2018年浙江省湖州市急性胃肠炎病例GⅠ型诺如病毒感染状况及其基因型别特征 被引量:11

Infection status and genotype characteristics of GⅠ norovirus in acute gastroenteritis cases in Huzhou, Zhejiang, 2017–2018
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摘要 目的了解浙江省湖州市急性胃肠炎病例中GⅠ型诺如病毒的感染状况及基因型别特征。方法收集2017年4月至2018年6月湖州市2家哨点医院诊断为急性胃肠炎病例的粪便标本1 259份。采用实时荧光定量反转录–聚合酶链式反应(real time RT-PCR)对提取的病毒RNA进行GⅠ和GⅡ型诺如病毒核酸检测。采用RT-PCR对GⅠ型阳性标本进行RdRp区和VP1区部分片段的扩增和测序。综合在线分型工具和系统进化分析的结果判定病毒基因型别。结果诺如病毒核酸阳性171份,阳性率13.58%。GⅡ型和GⅠ型诺如病毒的检出阳性率分别为11.83%(149/1 259)和2.70%(34/1 259),GⅡ型诺如病毒为检出的主要型别。GⅠ型诺如病毒主要检出于2018年上半年。2018年2-6月各月GⅠ型诺如病毒的检出阳性率均大于监测期间GⅠ型的平均检出阳性率。基因分型结果显示,2018年检出的GⅠ型诺如病毒包括GⅠ.P4-GⅠ.5、GⅠ.P2-GⅠ.2、GⅠ.P1-GⅠ.1、GⅠ.Pd-GⅠ.3、GⅠ.P4-GⅠ.4、GⅠ.P2-GⅠ.5。其中GⅠ.P4-GⅠ.5、GⅠ.P2-GⅠ.5和GⅠ.Pd-GⅠ.3重组型诺如病毒首次在湖州市检出。结论湖州市流行的GⅠ型诺如病毒基因型别多样,重组现象明显。应加强GⅠ型诺如病毒在当地的分子流行病学监测,以及时发现新的变异或重组株。 Objective To understand the infection status and genotype characteristics of GⅠ norovirus in acute gastroenteritis cases in Huzhou,Zhejiang province.Methods A total of 1 259 stool samples of acute gastroenteritis cases diagnosed in two sentinel hospitals in Huzhou from April 2017 to June 2018 were collected.The extracted viral RNA was subjected to GⅠ and GⅡ norovirus nucleic acid detections by using real-time quantitative reverse transcription polymerase chain reaction( RT-PCR).GⅠ positive samples were amplified and sequenced by RT-PCR in RdRp and VP1 regions.The genotyping of norovirus was confirmed by phylogenetic analysis and online typing tools.Results A total of 171 stool samples were norovirus nucleic acid positive,the positive rate was 13.58%.The positive rates of GⅡ and GI noroviruses were11.83%(149/1 259) and 2.70%(34/1 259),respectively,GⅡ was the main genotype detected.GⅠ norovirus was mainly detected in the first half of 2018.The positive detection rate of GⅠ norovirus in each month from February to June 2018 was higher than the average detection rate of GⅠ during the surveillance period.The genotyping results showed that the GⅠnorovirus detected in 2018 included GⅠ.P4-GⅠ.5,GⅠ.P2-GⅠ.2,GⅠ.P1-GⅠ.1,GⅠ.Pd-GⅠ.3,GⅠ.P4-GⅠ.4 and GⅠ.P2-GⅠ.5,in which GⅠ.P4-GⅠ.5,GⅠ.P2-GⅠ.5 and GⅠ.Pd-GⅠ.3 recombinant norovirus were detected for the first time in Huzhou.Conclusion The genotypes of GⅠ noroviruses circulating in Huzhou are diverse and the recombination phenomenon is obvious.It is necessary to strengthen the molecular epidemiological surveillance for GⅠ norovirus for the timely detection of variants or recombinant strains.
作者 纪蕾 祝永英 陈莉萍 沈月华 吴晓芳 徐德顺 Ji Lei;Zhu Yongying;Chen Liping;Shen Yuehua;Wu Xiaofang;Xu Deshun(Huzhou Prefectural Center for Disease Control and Prevention,Huzhou 313000,Zhejiang,China;Deqing County Center for Disease Control and Prevention,Huzhou 313200,Zhejiang,China)
出处 《疾病监测》 CAS 2019年第6期536-540,共5页 Disease Surveillance
基金 浙江省医药卫生科技计划(No.2018RC015) 湖州市自然科学基金(No.2017YZ04)~~
关键词 诺如病毒 胃肠炎 GⅠ型 基因分型 Norovirus Gastroenteritis Genogroup I Genotyping
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