苦荞提取物的关键香气成分分析

Identification of Key Odorants in Tartary Buckwheat Extracts

采用固相微萃取(SPME)结合气相色谱-质谱联用技术(GC-MS)分析苦荞提取物中的挥发性成分;同时采用气相色谱-嗅闻仪(GC-O)结合香气提取稀释分析(AEDA)确定其香气活性成分,通过对香气活性成分定量并计算其香气活性值(OAV)最终确定苦荞提取物的关键性香气成分。结果显示,SPME-GC-MS在苦荞提取物中共鉴定出92种挥发性成分,包括酯类28种、醛类7种、醇类9种、酮类7种、酸类6种、烯烃8种、烷烃20种、其他类7种;经GC-O嗅闻仪共鉴定出17种香气活性化合物,定量结果显示,苯甲醇(8637.40μg/kg),苯乙醛(3150.47μg/kg)、D-柠檬烯(3080.00μg/kg)含量较高;结合稀释因子(FD)和香气活性值(OAV)推测,苯甲醛(FD=81,OAV=5)、二甲基三硫(FD=243,OAV=48)、苯甲醇(FD=81,OAV=9)、苯乙醛(FD=729,OAV=787)、丙二酸二乙酯(FD=81,OAV=1.2)5种物质对苦荞提取物的风味贡献最大,是苦荞提取物的关键香气成分。

Volatile compounds of tartary buckwheat were extracted by solid phase micro-extraction(SPME)and analyzed by gas chromatography-mass spectrometry(GC-MS).Then,aroma-active compounds were identified by gas chromatography-olfactory(GC-O)combined with aroma extraction dilution analysis(AEDA).Finally,the key odorants of tartary buckwheat extracts were confirmed by their odor activity values(OAVs).The results showed that 92 kinds of volatile compounds including 28 esters,7 aldehydes,9 alcohols,7 ketones,6 acids,8 olefins,20 alkanes,and 7 others were identified by SPME-GC-MS.By application of GC-O/AEDA,17 odor-active compounds with flavor dilution(FD)factor range from 1 to243 were detected.Quantitation results showed that benzyl alcohol(8637.40μg/kg),phenylacetaldehyde(3150.47μg/kg),and D-limonene(3080.00μg/kg)had a higher concentration.It was speculated that benzaldehyde(FD=81,OAV=5),dimethyl trisulfide(FD=243,OAV=48),benzyl alcohol(FD=81,OAV=9),phenylacetaldehyde(FD=729,OAV=787),diethyl malonate(FD=81,OAV=1.2)were confirmed as the key odorants of tartary Buckwheat extracts for their highest FD factors and OAVs.