卵泡液中LH E2 P与女性年龄的相关性分析

Correlation Analysis between LH E2 and P in Follicular Fluid and Female Age

目的:通过检测不同年龄组不孕症患者卵泡液促黄体生成素(luteotropic hormone,LH)、雌二醇(Estradial,E2)、孕激素(Progesterone,P)水平,分析卵泡液LH、E2、P与女性年龄的相关性。方法:选择我院2017年7月至2018年6月期间收治的140例接受IVF-ET/ICSI助孕的患者作为研究对象,按照年龄分为A、B、C3个组,A组(<35岁,n=55),B组(35~39岁,n=55),C组(>39岁,n=30),利用ELISA检测取卵日卵泡液LH、E2、P水平,分析3组不孕症患者的一般资料和取卵日卵泡液LH、E2、P水平差异,比较不同年龄组间获卵数、受精率、可用胚胎率、优质胚胎率、临床妊娠率。结果:A组、B组、C组的一般资料中,BMI差异无统计学意义(P>0.05),但年龄、基础血清卵泡刺激素(bFSH)及窦卵泡(AFC)差异均有统计学意义(P均<0.05);A组、B组、C组的取卵日的卵泡液LH水平、E2水平差异均有统计学意义(P均<0.05),但3组取卵日的卵泡液P水平差异无统计学意义(P>0.05);经Spearman相关分析,取卵日的卵泡液LH水平与女性年龄呈正相关(r=0.335,P<0.05),E2水平与女性年龄呈负相关(r=-0.418,P<0.05),P水平与女性年龄无相关性(r=0.045,P=0.523);A组、B组、C组的获卵数、受精率、多PN受精率差异有统计学意义(P<0.05),但可用胚胎率、优质胚胎率、临床妊娠率差异无统计学意义(P>0.05)。结论:卵泡液LH、E2水平与年龄相关;卵巢的储备功能与年龄、bFsh及AFC有关,35~39岁为卵巢储备功能开始下降阶段,且卵巢反应性(通过获卵数反映)亦随之下降,尤其是39岁以上的妇女,卵巢储备功能减退明显,卵巢反应性也出现急剧下降。

Objective:To analyze the correlation between LH,E2 and P in follicular fluid and female age by measuring the levels of luteotropic hormone(LH),estradiol(E2)and progesterone(P)in follicular fluid of infertile women in different age groups.Methods:A total of 140 patients who received IVF-ET/ICSI assisted pregnancy from July 2017 to June 2018 were enrolled in the study.They were divided into groups A,B,and C according to age,and group A(<35 years old,n=55),group B(35-39 years old,n=55),group C(>39 years old,n=30),ELISA was used to detect the levels of LH,E2 and P in follicular fluid on day of egg retrieval,and three groups of infertility were analyzed.General information of patients with disease,the difference of LH,E2,P levels of follicular fluid on the day of egg retrieval and comparison of the number of eggs obtained in different age groups,fertilization rate,available embryo rate,high quality embryo rate,clinical pregnancy rate.Results:There were no significant differences in BMI between the general data of group A,group B and group C(P>0.05),but there were significant differences in age,basal serum follicle stimulating hormone(bFSH)and antral follicle(AFC).P<0.05);the difference of LH level and E2 level in follicular fluid of group A,group B and group C was statistically significant(P<0.05),but the level of follicular fluid P in the three groups was taken.The difference was not statistically significant(P>0.05).According to Spearman correlation analysis,the LH level of follicular fluid was positively correlated with female age(r=0.335,P<0.05),and E2 level was negatively correlated with female age(r=-0.418,P<0.05),there was no correlation between P level and female age(r=0.045,P=0.523).There were significant differences in the number of eggs,fertilization rate and multi-PN fertilization rate in group A,B and C.Significance(P<0.05),but there was no significant difference in available embryo rate,high quality embryo rate and clinical pregnancy rate(P>0.05).Conclusion:The levels of LH and E2 in follicular fluid are related to age.The ovarian reserve function is related to age,bFsh and AFC.The age of 35~39 years old is the beginning of the decline of ovarian reserve function,and the ovarian response(reflected by the number of eggs)is also followed.Decreased,especially in women over the age of 39,ovarian reserve dysfunction is evident,and ovarian response is also dramatically reduced.