IL-38在胃癌组织中的表达及临床意义

Expression and clinical significance of IL-38 protein in gastric carcinoma using tissue microarray

目的:探讨IL-38蛋白在胃癌组织中的表达及其与胃癌患者各临床病理参数之间的关系,探讨其与胃癌患者预后的关系。方法:建立组织芯片,通过免疫组织化学SP法检测78例无术前放、化疗史的胃癌患者手术标本和72例癌旁组织中IL-38蛋白的表达情况,并对随访患者进行生存分析。结果:IL-38在胃癌组织中阳性表达率为84.6%,在癌旁组织表达率为45.8%,差异有统计学意义(P<0.01);IL-38阳性表达与肿瘤TNM分期和淋巴结转移有关(P<0.05)。Kaplan-Meier生存分析结果显示,IL-38的高表达与胃癌不良预后有关(P<0.01)。经Cox比例风险回归模型分析显示,IL-38、肿瘤TNM临床分期和淋巴结转移与患者预后有关,且IL-38、有无淋巴结转移可作为独立的预后因素。结论:IL-38在胃癌组织中高表达,IL-38阳性表达与肿瘤TNM分期和淋巴结转移有关。IL-38高表达与胃癌患者不良预后相关,且IL-38、有无淋巴结转移可作为独立的预后因素。本实验明确IL-38与胃癌临床病理参数及预后之间的关系,为胃癌的临床诊断及治疗提供新的靶点。

Objective:To investigate the expression and significance of IL-38 protein in gastric carcinoma and normal tissues adjacent to carcinoma and to evaluate the relationship between the expressions and the clinical pathologic factors.Methods:High throughput technology of tissue microarray and immunohistochemical SP method were applied to detect the expressions of IL-38 protein in 78 gastric carcinoma specimens that did not have a history of chemo-radiotherapy and 72 non-tumor gastric tissues.The followed-up cases were used for prognosis analysis.Results:The positive expression rate of IL-38 in gastric cancer tissues was 84.6%,and the expression rate in adjacent tissues was 45.8%.The difference between the two groups was statistically significant(P<0.01).IL-38 protein showed relationship with tumor TNM stage and lymph node metastasis(P<0.05).Kaplan-Meier survival analysis showed that the high expression of IL-38 was associated with poor prognosis of gastric cancer(P<0.01).Cox proportional hazard regression analysis showed that IL-38,tumor TNM clinical stage and lymph node metastasis were associated with prognosis,and IL-38 and lymph node metastasis could be used as independent prognostic factors.Conclusion:IL-38 is highly expressed in gastric cancer tissues,and the positive expression of IL-38 is associated with tumor TNM stage and lymph node metastasis.High expression of IL-38 is associated with poor prognosis in patients with gastric cancer,and IL-38 and lymph node metastasis can be used as independent prognostic factors.This experiment clarifies the relationship between IL-38 and clinicopathological parameters and prognosis of gastric cancer,and provides a new target for clinical diagnosis and treatment of gastric cancer.