miR-155在鼻咽癌中的表达及对人鼻咽癌细胞增殖的影响

Expression of miR-155 in nasopharyngeal carcinoma and its effect on proliferation of human nasopharyngeal carcinoma cells

目的:探究miR-155(microRNA-155)在鼻咽癌中的表达及对人鼻咽癌细胞增殖的影响。方法:选取于本院行手术切除治疗的鼻咽癌患者,利用实时荧光定量PCR检测miR-155在鼻咽癌组织、正常组织以及在鼻咽癌细胞系CEN1、正常鼻咽上皮细胞NP69中的表达量,沉默miR-155,检测其对人鼻咽癌细胞增殖的影响。结果:miR-155在鼻咽癌癌组织、细胞系CEN1中的表达量显著高于正常组织、鼻咽上皮细胞NP69中的表达量(t=8.560,P=0.000;t=42.386,P=0.000);鼻咽癌患者组织中miR-155表达与肿瘤TNM分期、病理分级、浸润范围以及淋巴结是否转移相关(P<0.05),与患者年龄、性别无关(P>0.05);转染miR-155-inhibitor后miR-155表达量较对照组与空白对照组明显下降,差异具有统计学意义(F=35.63,P=0.003);沉默miR-155,鼻咽癌细胞的生长速度明显慢于对照组与空白对照组(P<0.05)。结论:miR-155在人鼻咽癌组织与细胞中高表达,抑制其表达,可抑制鼻咽癌细胞的增殖,其对于鼻咽癌的治疗具有重要意义。

Objective:To investigate the expression of miR-155(microRNA-155)in nasopharyngeal carcinoma(NPC)and its effect on the proliferation of nasopharyngeal carcinoma cells.Methods:From September 2016 to September 2018,Patients with nasopharyngeal carcinoma who underwent surgical resection were selected,and the expression of miR-155 in nasopharyngeal carcinoma(NPC)tissues,normal tissues and nasopharyngeal carcinoma cell lines(CEN1),and the expression of NP69 in normal nasopharyngeal epithelial cells was detected by real-time fluorescence quantitative PCR.The effect of silencing miR-155 on the proliferation of human nasopharyngeal carcinoma cells was detected.Results:The expressions of miR-155 in nasopharyngeal carcinoma tissue and cell line CEN1 were significantly higher than that in normal tissues.The expression level of NP69 in nasopharyngeal epithelial cells was significantly higher than that in nasopharyngeal epithelial cells(t=8.560,P=0.000;t=42.386,P=0.000).The expression of miR-155 in nasopharyngeal carcinoma was correlated with TNM stage,pathological grade,extent of invasion and lymph node metastasis(P<0.05),but not with age and sex(P>0.05).The expression of miR-155 after transfection of miR-155-inhibitor was significantly lower than that of the control group and the blank control group(F=35.63,P=0.003).The growth rate of NPC cells silenced by miR-155,was significantly slower than that of the control group and the blank control group(P<0.05).Conclusion:The high expression of miR-155 in human nasopharyngeal carcinoma tissues and cells can inhibit the proliferation of nasopharyngeal carcinoma cells,and it is of great significance for the treatment of nasopharyngeal carcinoma.