10个冷季型草坪草品种在高温胁迫下的抗性

Differences in resistance to high temperature stress of 10 cold-season turfgrass varieties

为筛选适合湖南地区的冷季型草坪草种,解决冷季型草坪草在湖南地区越夏的问题,选用了湖南地区适应性较好的高羊茅(Festuca arundinacea)、紫羊茅(Festuca rubra)、黑麦草(Lolium pernne)、早熟禾(Poa pratensis)、白三叶(Trifolium repens)和匍匐剪股颖(Agrostis stolonifera)6个不同冷季型草种的10个不同品种为研究对象,分析了它们在草坪建植后的坪用性状以及在25(CK)和30、35、40℃三个高温胁迫下的叶片游离脯氨酸含量、可溶性糖含量、叶绿素含量、丙二醛含量、相对电导率5项生理生化指标的变化。结果表明,10个不同品种的坪用性状与高温胁迫下的各生理生化指标变化存在着明显的种间或品种间差异。高温胁迫下,叶片的可溶性糖含量、丙二醛含量、游离脯氨酸含量和相对电导率随温度的升高而增加,而叶绿素含量随温度的升高而降低,且这5个指标分属冷季型草高温抗性的两大类型,分别为抗高温损害能力、逆境调节能力。根据不同冷季型草坪草品种田间种植的坪用性状的表现和高温胁迫下各生理生化指标变化综合分析得出,高温胁迫下不同冷季型草坪草种的高温抗性表现为高羊茅维加斯>高羊茅锐步>紫羊茅桥港Ⅱ>高羊茅易凯>白三叶考拉>早熟禾百思特>黑麦草百舸>黑麦草爱神特>早熟禾威罗>匍匐剪股颖摄政王。

To screen the suitability of cold-season turfgrass varieties for Hunan and solve their problems associated with summer in Hunan,10 cold-season turfgrass cultivars of six species adapting well and widely naturalized in Hunan were selected as research objects,namely,Festuca arundinacea,Festuca rubra,Lolium perenne,Poa pratensis,Trifolium repens,and Agrostis stolonifera.The changes in their turf characteristics;physiological and biochemical indicators of free proline,soluble sugar,chlorophyll,and malondialdehyde content;and leaf membrane lipid permeability at 25℃(CK)and three stress-related high temperatures,30℃,35℃,and 40℃were analyzed.The results showed no obvious differences in turf characteristics and physiological and biochemical indicators between the 10 cold-season turfgrass cultivars of the six species under high-temperature stress.The soluble sugar,malondialdehyde,and proline content in the leaves and leaf electric conductivity increased with increasing temperature.The chlorophyll content decreased with increasing temperature.According to the turf characteristics expressed in the field planting experiment and the physiological and biochemical index changes under high-temperature stress of the different cold-season turfgrass cultivars,their high-temperature resistance was concluded to be in the following order:Festuca arundinacea‘Vegas’>Festuca arundinacea‘Barrera’>Festuca rubra‘Bridgeport Ⅱ’>Festuca arundinacea‘Ikay’>Trifolium repens‘Koala’>Poa pratensis‘Barrister’>Lolium perenne‘Bartwingo’>Lolium perenne‘Accent’>Poa pratensis‘Wellow’>Agrostis stolonifera‘Regent’.