NaCl处理对黑果枸杞叶解剖结构的影响

Effect of NaCl treatment on the anatomy of Lycium ruthenicum leaves

以黑果枸杞(Lycium ruthenicum)为研究对象,在不同浓度NaCl处理下,观察黑果枸杞叶片形态特征的变化,采用石蜡切片技术研究叶片解剖结构的变化,分析各参数间的差异及与NaCl处理之间的关系。结果表明,随着NaCl浓度的增大,黑果枸杞叶厚、栅栏组织厚度和中脉厚度也随之增大,表皮细胞厚度呈先增大后减小的趋势,海绵组织厚度呈下降趋势,表现出贮盐植物典型特征。当NaCl浓度为100 mmol·L^–1时,处理15 d时上下表皮厚度和叶片组织结构紧密度分别为67.72μm、44.39μm和29.90%,处理30 d时分别为79.70μm、55.99μm和33.54%;当NaCl浓度继续增加,上下表皮厚度和叶片组织结构紧密度呈下降趋势,其余指标无明显的规律性变化。黑果枸杞可在NaCl浓度为100 mmol·L^–1以下时正常生长,当盐浓度继续增加时,其生长受到抑制。

The present study investigated the effects of NaCl treatment on the anatomy of Lycium ruthenicum leaves.The leaf anatomy was studied using paraffin sectioning,and the relationship between NaCl concentration and various anatomical parameters were analyzed.The results confirmed that L.ruthenicum is a typical salt-tolerant plant that possesses salt-resistant structures.With increasing NaCl concentration,the thickness of the leaves,palisade parenchyma and main veins increased,the thickness of the epidermis increased initially and then decreased,and the thickness of the spongy parenchyma decreased.When the NaCl concentration was 100 mmol·L^–1,the thicknesses of the upper and lower epidermises and the cell tense ratio of the leaf after 15 days were 67.72μm,44.39μm,and 29.90%,respectively,and after 30 days were 79.70μm,55.99μm,and 33.54%,respectively.However,with increasing NaCl concentrations,the thickness of the upper and lower epidermises and the CTR decreased.Meanwhile,no significant or regular changes were observed in the other indicators.Therefore,L.ruthenicum can grow normally at NaCl concentrations below 100 mmol·L^–1 but is adversely affected by higher concentrations.