摘要
建立了测定药物及生物样品中吡哌酸的单波长和双波长可见吸收光谱法。在可见光区和pH6.63Tris-HCl介质中,吡哌酸与碱性桃红反应生成红色二元离子缔合物,产生一个较强的正吸收峰和一个较强的负吸收峰,最大正吸收峰位于576nm,最大负吸收峰位于522nm,在此二波长处,两单波长法的线性范围均为0.02~3.7mg·L^-1,表观摩尔吸光系数(κ)分别为4.37×10^4L·mol^-1·cm^-1 和1.08×10^4L·mol^-1·cm^-1。当用双波长法测定时,线性范围为0.02~3.7mg·L^-1,表观摩尔吸光系数(κ)为5.45×10^4L·mol^-1·cm^-1。将576nm处的单波长法用于药物中吡哌酸含量的测定,回收率和相对标准偏差(n=5)分别为97.5%~102%和2.5%~2.8%。
A new single-wavelength and double-wavelength visible absorption spectrometry for the determination of pipemidic acid in drug and biological sample was established.In a Tris-HCl medium of pH 6.63 and in the visible region,pipemidic acid reacted with alkaline pink to form a red binary ion association complexes,as a result,a strong positive absorption peak and a strong negative absorption peak were produced.The largest positive absorption peak was located at 576 nm and the largest negative absorption peak was located at 522 nm.At the two wavelengths,Their linear range of two single wavelength method were 0.02~3.7 mg·L^-1.The apparent molar absorbance coefficient(κ)were 4.37×10^4 L·mol^-1·cm^-1 and 1.08×10^4 L·mol^-1·cm^-1,respectively.When the double-wavelength absorption spectrometry was used to determine the concentrations of pipemidic acid,its linear range was 0.02~3.7 mg·L^-1,the apparent molar absorbance coefficient(κ)was 5.45×10^4 L·mol^-1·cm^-1.The single-wavelength method at 576 nm was applied to the determination of pipemidic acid in drug with the recovery rate and the relative standard deviation(n=5)were 97.5%~102%and 2.5%~2.8%,respectively.
作者
次晓曼
江虹
谭茜
CI Xiao-man;JIANG Hong;TAN Qian(College of Chemistry and Chemical Engineering,Collaborative Innovation Center for Green Development in Wuling Mountain Areas,Yangtze Normal University,Chongqing 408100,China)
出处
《化学研究与应用》
CAS
CSCD
北大核心
2019年第7期1368-1372,共5页
Chemical Research and Application
基金
重庆市教委科技基金项目(KJ1401226)资助
长江师范学院科技基金项目(2018XKY096)资助
关键词
吡哌酸
碱性桃红
单波长
双波长
吸收光谱法
pipemidic acid
alkaline peach pink
single-wavelength
double-wavelength
absorption spectrometry