摘要
为了解田间感赤星病烟叶的叶际真菌群落结构,提取感病烟叶样品DNA,扩增子测序法扩增样品的ITS区,采用IonS5XL高通量测序技术对扩增片段进行高通量测序,并对其真菌群落结构进行分析。结果表明:田间感赤星病烟叶叶际真菌群落结构较为典型;门水平包括子囊菌门(Ascomycota 86.49%)、担子菌门(Basidiomycota 0.61%)及壶菌门(Chytridiomycota 0.01%);属水平包括链格孢属(Alternaria 83%)、茎点霉属(Phoma 1.43%)、镰刀霉属(Fusarium 0.79%、Symmetrospora 0.45%)、赤霉菌属(Gibberella 0.33%)、Hannaell 0.11%)、粉红螺旋聚孢霉(Clonostachys 0.09%)、枝孢霉属(Cladosporium 0.15%、Stagonosporopsis 0.07%、Plectosphaerella 0.07%)。
To analyze the fungal community structure of leaf phyllosphere from field tobacco infected which with brown spot,DNA was extracted from diseased tobacco leaves,and ITS region was amplified and sequenced.IonS5XL high throughput sequencing technique was used to analyze the structure of fungal community.Results:Fungal community structure of leaf phyllosphere from tobacco brown spot disease was quite typical.At the phylum level,the fungi included Ascomycota(86.49%),Basidiomycota(0.61%)and Chytridiomycota(0.01%),while at the genus level,the fungi included Alternaria(83%),Phoma(1.43%),Fusarium(0.79%),Symmetrospora(0.45%)and Gibberella(0.33%),Hannaell(0.11%),Clonostachys(0.09%),Cladosporium(0.15%),Stagonosporopsis(0.07%)and Plectosphaerella(0.07%).
作者
刘畅
汪汉成
谢红炼
陈乾丽
余知和
孙光军
LIU Chang;WANG Hancheng;XIE Honglian;CHEN Qianli;YU Zhihe;SUN Guangjun(College of Life Sciences,Yangtze University,Jingzhou,Hubei 434025;Guizhou Academy of Tobacco Science,Guiyang,Guizhou 550081;College of Agriculture,Yangtze University,Jingzhou,Hubei 434025;College of Agriculture,Guizhou University,Guiyang,Guizhou 550025;Guizhou Provincial Tobacco Monopoly Bureau,Guiyang,Guizhou 550003,China)
出处
《贵州农业科学》
CAS
2019年第7期54-59,共6页
Guizhou Agricultural Sciences
基金
国家自然科学基金项目“基于表型组学的嘧菌酯对烟草赤星病菌的作用机理及其抗性机理研究”（31360448）
贵州省科技厅优秀青年人才培养计划项目“集约化育苗下烟草立枯病的流行规律及其防控技术研究”[黔科合平台人才（2017）5619]
中国烟草总公司贵州省公司科技项目“‘’互联网+烟草植保’的研究与应用”（201714），“贵州烟草叶斑类病害成灾规律与绿色防控关键技术研究示范”（2019020）
