Po蛋白促进正己烷中毒性周围神经病患者外周血INF-v和IL-10的表达

Po protein promotes the expression of INF-7 and IL-10 in peripheral blood of n-hexane neuropathypatients

目的通过研究P。蛋白引发的正己烷中毒性周围神经病患者外周血单核细胞因子的变化.探讨自身免疫在正己烷周围神经病中的作用方法于2018年5月,选择20 17年在深圳市职业病防治院住院,并诊断为正己烷中毒周围神经病的5 名患者作为病例组,选择6 名正己烷接触工人、6名未接触正己烷工人分别作为接触组、对照组。采集调查对象静脉血,分离外周血单个核细胞(Peripheral blood mononuclear cell, PBMC),米用酶联免疫斑点试验(Enzyme-linked Immunospot Assay,ELISPOT)检测PBMC经P°蛋白、植物血球凝集素(Phytohemagglutinin, PHA )处理后PBMC 干扰素-丫(INF-y )和细胞因子10(IL-10啲分泌情况。结果与对照组和接触组比较,病例组PBMC经P0 蛋白抗原或PHA 处理后.INF-7 JL-10产生的抗原特异性T 淋巴细胞(SFCs)数均升高,差异有统计学意义(P<0.01 )0与对照组比较,接触组PBMC 经PHA 处理后.INF-7JL-10产生的SFCs 数均升高,差异有统计学意义(P<0.05),接触组PBMC 经P 。蛋白抗原处理后,INF-7.IL-10产生的SF Cs数无明显变化,差异无统计学意义(60.05)。结论P 。蛋白引发的自身免疫可能参与了正己烷中毒周围神经病髓鞘损害的发生。

Objective To study the changes of monocyte cytokines in peripheral blood of n-hexane neuropathy patients induced by Po protein, and to explore the role of autoimmunity in n-hexane neuropathy patients. Methods In May 2018, 5 patients with peripheral neuropathy diagnosed as n-hexane poisoning were selected as case group in Shenzhen Prevention and Treatment Center for Occupational Disease in 2017. 6 workers exposure to n-hexane and 6 workers without n-hexane exposure were selected as contact group and control group. Peripheral blood mononuclear cells (PBMC) were isolated from venous blood. Results The number of spots produced by INF-7 and IL-10 increased after stimulation with Po protein in case group, and the positive rate was significantly higher than control group and the contact group. Conclusion Autoimmunity induced by Po protein may be involved in the occurrence of myelin sheath damage in n-hexane neuropathy patients.