VBNC菌群中好氧反硝化菌种的复苏培养及其脱氮特性

Resuscitation of aerobic denitrifying bacteria in the VBNC state and their nitrogen conversion capacity

高氨氮废水的生物处理技术因安全高效而备受关注,但反硝化细菌的分离菌源仅限于常规分离培养法所获得的不到1%的微生物,绝大多数菌株因处于活的但非可培养(“viable but non-culturable”,VBNC)状态而未能被发掘。复苏培养VBNC菌群对强化高氨氮废水的生物处理具有重要意义。本文利用复苏促进因子复苏培养高氨氮废水中的VBNC菌株,16S rRNA基因测序对其进行同源性分析,初步鉴定复苏培养的菌株归属于Gordonia和Pseudomonas属。通过测定特殊培养基中硝态氮、亚硝态氮和氨氮的浓度,研究VBNC菌株的好氧反硝化性能,其中,菌株ZYR51的好氧反硝化能力较强。本研究为挖掘潜在具有好氧反硝化性能的VBNC菌种提供了新方法,并为筛选高效去除高氨氮废水的菌种资源提供了新思路。

At the present,the biological treatment of high ammonium nitrogen wastewater has attracted much attention because of its environmental friendly characteristics and high efficiency.Most of strains can not be excavated due to entry into a viable but non-culturable(VBNC)state.The resuscitation of VBNC strains is important for biological treatment of wastewater with high ammonium nitrogen.In this study,VBNC strain was cultured in high ammonium nitrogen wastewater by resuscitation-promoting factors(Rpf)resuscitation,and its homology was analyzed by 16S rRNA gene sequencing.The isolated strains,which were originally in the VBNC state,were further conducted to investigate the aerobic denitrification capacities with the help of the analyses of nitrate,nitrite,total nitrogen,and ammonium nitrogen.The results showed that the strains belonged to Gordonia and Pseudomonas,among which the aerobic denitrification ability of strain ZYR51 was the highest.This study provides a new method for excavation of VBNC bacteria with aerobic denitrifying properties and a new way for screening efficient strains in the treatment of ammonium nitrogen wastewater.