全身振动训练联合正弦交变电磁场对去卵巢骨质疏松大鼠骨密度、骨代谢指标的影响研究

Effects of whole body vibration training combined with sinusoidal alternating electromagnetic field on bone mineral density and bone metabolism in ovariectomized osteoporotic rats

目的探讨全身振动训练联合正弦交变电磁场对去卵巢骨质疏松大鼠骨密度、骨代谢、骨生物力学性能的影响。方法将100只大鼠随机分为切除卵巢组和对照组,分别进行卵巢切除术和假手术,大鼠进行6周恢复,恢复后将造模成功的切除卵巢大鼠随机分为模型组(MO组)、全身振动训练组(W组)、正弦交变电磁场组(S组)、全身振动训练+正弦交变电磁场组(WS组),对照组列为假手术组(SO组),进行为期16周干预,干预结束后对大鼠进行骨密度、骨代谢、骨生物力学性质的检测。结果16周干预完成后,MO组、S组大鼠体质量显著高于SO组、W组、WS组大鼠(P<0.05);W组、WS组、SO组大鼠骨密度指标、血清雌二醇浓度指标明显高于大鼠MO组(P<0.05);SO组大鼠血清雌二醇浓度指标明显高于S组、W组、WS组(P<0.05);M组大鼠血清OC、ALP浓度明显高于SO组、W组、S组、WS组大鼠(P<0.05);尿液DPD/Cre、Ca/Cre、P/Cre浓度方面,M组大鼠明显高于SO组、W组、S组、WS组大鼠(P<0.05)。SO组、W组、S组、WS组大鼠股骨最大载荷和弹性模型明显高于MO组大鼠(P<0.05)。SO组、W组、S组、WS组大鼠股骨断裂载荷组间无明显差异(P>0.05);SO组、W组、S组、WS组大鼠股骨弹性模量明显高于MO组大鼠(P<0.05)。SO组大鼠股骨弹性模量明显高于S组大鼠(P<0.05),但与W组、WS组大鼠没有明显差异(P>0.05)。L4椎体压缩试验,SO组、W组、S组、WS组大鼠股骨最大载荷和弹性模型明显高于MO组大鼠(P<0.05)。SO组大鼠高于S组、W组,差异有统计学意义(P<0.05),但与WS组大鼠没有明显差异(P>0.05)。结论全身振动训练、正弦交变电磁场、全身振动训练联合正弦交变电磁场3种干预方式施用于去卵巢骨质疏松大鼠均能提升骨密度、抑制骨吸收、平衡骨代谢、改善骨骼结构力学和材料力学性能。而全身振动训练联合正弦交变电磁场能的治疗效果优于单纯使用全身振动训练或正弦交变电磁场,在临床应用中有一定推广价值。

Objective To investigate the effects of whole body vibration training combined with sinusoidal alternating electromagnetic field on bone mineral density,bone metabolism and bone biomechanical properties in ovariectomized osteoporotic rats.Methods One hundred rats were randomly divided into the ovariectomized group and the control group.Ovariectomy and sham operation were performed respectively.The rats were recovered for 6 weeks.After recovery,the successfully ovariectomized rats were randomly divided into model groups( MO group),whole body vibration training group( W group),sinusoidal alternating electromagnetic field group( S group) and whole body vibration training + sinusoidal alternating electromagnetic field group( WS group),and the control group was classified as sham operation group( SO group).The intervention was performed for 16 weeks.After the intervention,the rats were tested for bone mineral density,bone metabolism and bone biomechanical properties.Results After 16 weeks of intervention,the body weight of the rats in the MO group and the S group was significantly higher than that in the SO group,W group and WS group( P<0.05);bone density index and serum estradiol concentration in the W group,WS group and SO group were significantly higher than that of the MO group( P<0.05).The serum concentration of estradiol in the SO group was significantly higher than that in the S group,W group and WS group( P<0.05).Serum concentration of OC and ALP in the M group was significantly higher than that of SO group,W group,S group and WS group( P<0.05).For urinary DPD/Cre,Ca/Cre and P/Cre concentration,M group rats had significantly higher levels than rats in SO group,W group,S group and WS group( P<0.05).The maximal femoral load and elasticity model of rats in SO group,W group,S group and WS group were significantly higher than those in MO group( P< 0.05).There was no significant difference in femoral fracture load between SO group,W group,S group and WS group( P > 0.05).The femoral elastic modulus of SO group,W group,S group and WS group was significantly higher than that of MO group( P<0.05).The femoral elastic modulus of the rats in the SO group was significantly higher than that in the S group( P<0.05),but was not significantly different from that in the W group and the WS group( P>0.05).In the L4 vertebral compression test,the maximal femoral load and elasticity model of SO group,W group,S group and WS group were significantly higher than those of MO group( P<0.05);rats in the SO group had significantly higher values than those in the S group and the W group( P< 0.05),but was not significantly different from rats in the WS group( P> 0.05).Conclusion Whole body vibration training,sinusoidal alternating electromagnetic field,whole body vibration training combined with sinusoidal alternating electromagnetic field can be applied to ovariectomized osteoporotic rats to increase bone density,inhibit bone resorption,balance bone metabolism,and improve bone structure and mechanical properties of materials.Whole body vibration training combined with sinusoidal alternating electromagnetic field energy is better than the simple use of whole body vibration training or sinusoidal alternating electromagnetic field,thus has certain promotion value in clinical application.