猪伪狂犬病病毒与副猪嗜血杆菌混合感染的综合诊断及感染毒株gE基因遗传进化分析

Comprehensive Diagnosis of Mixed Infection with Porcine Pseudorabies Virus and Haemophilus parasuis and Phylogenetic Analysis on the gE Gene of Infected Strain

2019年4月,山东省新泰市某育肥猪场从某种猪场购进了100头5周龄仔猪。购进1周后,有40多头仔猪表现昏睡、呕吐、拉稀等症状并出现急性死亡,共死亡28头,病死率高达68%。对病猪进行病理剖检、组织病理学检查、病原学检测等实验室综合诊断,最终确诊为伪狂犬病病毒和副猪嗜血杆菌的混合感染。对分离的伪狂犬病病毒gE基因进行测序和遗传进化分析,确定该分离株与近期流行株同源性较高,这为该病疫苗的选择和高效使用提供了依据。基于诊断结果,制定了相应的防控措施,使疫情得到了迅速控制。本文还分析讨论了该病例的发生原因、发病特点及病变特征,对类似病例的诊断防控具有一定的指导意义。

In April 2019,100 piglets of 5-week old were purchased from a breeding farm by a fattening farm in Xintai City of Shandong Province.Subsequently,some symptoms such as lethargy,vomiting,diarrhea or even death appeared in more than 40 piglets after one week,a total of 28 piglets died,with a mortality rate of 68%,which was finally confirmed as the mixed infection with pseudorabies virus and Haemophilus parasuis through laboratory comprehensive diagnosis including pathological autopsy,histopathological examination and etiological test.Then the gE gene of isolated pseudorabies virus was sequenced and analyzed,and high homology of the isolate with current epidemic strain was found,which provided a basis for selection and efficient application of the vaccine.Based on the above results,corresponding control measures were formulated,and the outbreak was rapidly controlled.Furthermore,the reasons,clinical characters and pathological changes of the cases were discussed and analyzed in the paper,which were of significance for diagnosis and control of similar cases.