国产Bt-Cry1Ab和Bt-(Cry1Ab+Vip3Aa)玉米对草地贪夜蛾的抗性测定

The bioassay of Chinese domestic Bt-Cry1Ab and Bt-(Cry1Ab+Vip3Aa) maize against the fall armyworm, Spodoptera frugiperda

草地贪夜蛾Spodoptera frugiperda(J.E.Smith)是世界性重大农业害虫。种植转基因Bt作物是主要的防治手段之一。我们利用室内生物测定方法评价了国产Bt-Cry1Ab玉米(转化体C0030.3.5)和Bt-(Cry1Ab+Vip3Aa)玉米(转化体DBN3601和DBN3608)对草地贪夜蛾1~4龄幼虫的毒力。结果显示,供试两种转基因玉米共6个品种皆可高效表达目标杀虫蛋白并对草地贪夜蛾具有很强的毒杀作用,对1龄幼虫的致死率达到59%~100%,存活幼虫的生长发育亦受到显著抑制。表明国内研发的Bt-Cry1Ab玉米和Bt-(Cry1Ab+Vip3Aa)玉米对草地贪夜蛾具有良好的控制效果,其中Bt-(Cry1Ab+Vip3Aa)玉米的防治效果显著优于Bt-Cry1Ab玉米,两种转基因玉米皆具有较好的商业化应用前景。

The fall armyworm,Spodoptera frugiperda(J.E.Smith)is an important agricultural pest in the world and planting Bt crop has been widely deployed for the pest control in its native region,the American continent.In this study,the control efficacy of Chinese domestic Bt-Cry1Ab maize(Event-C0030.3.5)and Bt-(Cry1Ab+Vip3Aa)maize(Event-DBN3601 and DBN3608)to 1st-4th instar larvae of fall armyworm were evaluated by the method of feeding on maize leaves in the lab.The results showed that all the six varieties of two kinds Bt corns could express the target insecticidal proteins efficiently and have strong toxicity to the pest.The insecticidal efficacy to the 1st instar larvae reached 59%-100%,and the growth and development of the survived larvae were also significantly inhibited.Our results indicated that Bt-Cry1Ab and Bt-(Cry1Ab+Vip3Aa)maize developed in China had high control efficacy against fall armyworm.However,compared with the single Bt gene maize,the control efficacy of the two Bt gene maize was significantly higher.It is concluded that the two Bt maize present good commercial application prospects.