26个桑树品种的ITS序列位点的克隆与分析

Cloning of and Analysis on ITS in 26 mulberry Varieties

选择26个代表性的桑树品种,提取DNA作为模板,进行PCR反应,以已经公布的ITS引物作为本研究的扩增引物,同时从NCBI中搜索所有关于桑树的ITS扩增序列,下载与本研究的扩增序列进行比对。结果表明:26个代表性桑树品种中,只有毛桑出现了两个个变异位点,其它25个品种或种质序列完全一致,从Genbank中共下载到15条桑树的ITS序列,其中有3条序列含有3个变异位点。本研究证明,单独的ITS只能进行少数几个桑树品种的鉴别,不能完全准确地进行桑树品种分类。

The PCR reactions were carried out based on the DNA from 26 mulberry varieties.The primer of ITS which predecessors designed were synthesized.ITS sequences from NCBI were downloaded and aligned with 26 DNA ampliconic sequences.The results showed there were two variable sites in Maosang,the others had no variable site.There were three different variable sites distributed in 15 ITS downloaded from Genbank.Thus,it was difficult to identify mulberry varieties,although it can distinguish several varieties from others due to the single ITS.