摘要
利用羧基化氧化石墨烯(GO-COOH)的纳米酶效应,建立了一种谷胱甘肽制剂比色法含量测定方法。对反应时间、pH、H2O2浓度进行了优化。在最优条件下,谷胱甘肽标准品检测的线性范围为1~50μmol/L。将该方法用于谷胱甘肽片的含量测定,并采用液相色谱法对测定结果进行了验证,结果显示,谷胱甘肽片剂测定的相对误差为1.8%。该方法简单、直观,可用于谷胱甘肽制剂含量的准确测定。
A new method for the determination of glutathione content was established by using the nanoenzyme effect of carboxylated graphene oxide( GO-COOH). The reaction time,pH,and H2 O2 concentration were optimized.Under optimal conditions,the linear range of glutathione( GSH) standards was 1 ~ 50 μmol/L. The method was applied to the determination of practical sample,and verified by high performance liquid chromatography. The results showed that the content of glutathione tablets was easily and accurately measured with the relative error of 1. 8%,indicating that the method is simple and intuitive,and can accurately measure the content of glutathione tablet.
作者
董旭
郭华
闾春林
申刚义
Dong Xu;Guo Hua;Lv Chunlin;Shen Gangyi(Key Laboratory of Ethnomedicine ( Minzu University of China),Ministry of Education,School of Pharmacy,Minzu University of China,Beijing 100081)
出处
《化学通报》
CAS
CSCD
北大核心
2019年第7期659-662,共4页
Chemistry
基金
国家自然科学基金项目(81573834)
民族医药教育部重点实验室(中央民族大学)自主课题(KLEM-ZZ201808)
中央民族大学青年学术团队引领计划(2019QNYL26)资助
关键词
谷胱甘肽
含量测定
纳米酶
羧基化石墨烯
Glutathione
Assay
Nanoparticle enzyme
Carboxylated graphene oxide
