核仁纺锤体相关蛋白1在非小细胞肺癌中的表达及其与患者预后的关系

Expression of NUSAP1 and its relationship with prognosis in non-small cell lung cancer

目的探讨核仁纺锤体相关蛋白1(NUSAP1)在非小细胞肺癌中的表达及其与患者预后的关系。方法收集非小细胞肺癌及癌旁组织标本各50例,采用实时荧光定量PCR检测NUSAP1 mRNA的表达,采用免疫组化法检测NUSAP1蛋白的表达,采用在线数据库分析NUSAP1的表达及其与非小细胞肺癌患者预后的关系。结果肺癌组织中NUSAP1 mRNA的表达水平高于癌旁组织(P<0.05);NUSAP1蛋白在癌组织中的表达率为58.0%(29/50),在癌旁组织中的表达率为22.0%(11/50),差异有统计学意义(P<0.05)。NUSAP1蛋白的表达与患者的年龄和性别均无关(均P>0.05),但与肿瘤大小、淋巴结转移和TNM分期均有关(均P<0.05)。在线数据库分析显示,NUSAP1 mRNA的表达水平与患者的总生存时间(OS)有关(P<0.001);与NUSAP1高表达的患者比较,低表达患者的OS较长。Kaplan-Meier plotter在线数据库分析显示,NUSAP1 mRNA的表达与患者病理分级、临床分期、性别、化疗、吸烟史和组织分型均有关(均P<0.05)。结论NUSAP1在非小细胞肺癌组织中过表达,与肿瘤的生长发展和患者的预后有关,可作为非小细胞肺癌患者潜在的预后标志物。

Objective To investigate the expression of nucleolar and spindle-associated protein 1(NUSAP1)in non-small cell lung cancer(NSCLC)and analyze its relationship with the prognosis of NSCLC patients.Methods Real-time fluorescent quantitative PCR and immunohistochemical staining were performed to determine the expression of NUSAP1 in NSCLC tissues and adjacent tissues collected from hospital.The relationship between NUSAP1 expression and prognosis of NSCLC patients was analyzed by online database.Results The expression level of NUSAP1 mRNA in tumor tissues was significantly higher than that of adjacent tissues(P<0.05).The high expression rate of NUSAP1 protein in NSCLC tissues was 58.0%(29/50),significantly higher than 22.0%(11/50)of adjacent tissues(P<0.05).The high expression of NUSAP1 protein in NSCLC tissues was closely correlated with tumor size,lymph node metastasis and TNM stage(P<0.05),but was not related to age and gender.The data showed that the expression level of NUSAP1 mRNA was inversely associated with the overall survival(OS)of NSCLC patients(P<0.001).The expression of NUSAP1 mRNA was significantly correlated with the pathological grade,clinical stage,gender,chemotherapy,smoking history,and histological type of NSCLC patients(P<0.05).Conclusions The expression of NUSAP1 is up-regulated in NSCLC,which is correlated with the growth and development of NSCLC and prognosis of the patients.These results indicate that NUSAP1 can be used as a potential prognostic marker for NSCLC.